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Propofol, an anesthetic possessing neuroprotective action against oxidative stress, promotes the process of cell death induced by H2O2 in rat thymocytes

Published on Jul 1, 2006in European Journal of Pharmacology3.17
· DOI :10.1016/j.ejphar.2006.04.037
Ken-ichi Chikutei2
Estimated H-index: 2
(Tokushima Bunri University),
Tomohiro M. Oyama11
Estimated H-index: 11
(Tokushima Bunri University)
+ 7 AuthorsYasuooyama30
Estimated H-index: 30
(University of Tokushima)
Abstract
Abstract Propofol (2,6-diisopropylphenol) is a general anesthetic possessing a neuroprotective action against oxidative stress produced by H 2 O 2 . H 2 O 2 induces an exposure of phosphatidylserine on outer surface of cell membranes, resulting in change in membrane phospholipid arrangement, in rat thymocytes. Since propofol is highly lipophilic, the agent is presumed to interact with membrane lipids and hence to modify the cell vulnerability to H 2 O 2 . Therefore, to test the possibility, we have examined the effect of propofol on rat thymocytes simultaneously incubated with H 2 O 2 . Although propofol (up to 30 μM) alone did not significantly affect the cell viability, the agent at 10 μM started to increase the population of dead cells in the presence of 3 mM H 2 O 2 and the significant increase was observed at 30 μM. Propofol at clinically relevant concentrations (10–30 μM) facilitated the process of cell death induced by H 2 O 2 in rat thymocytes. However, propofol protected rat brain neurons against the oxidative stress induced by H 2 O 2 under same experimental condition. Therefore, the action of propofol may be dependent on the type of cells.
  • References (36)
  • Citations (12)
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References36
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#2Aimi Kanada (University of Tokushima)H-Index: 6
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Abstract We have previously reported that cremophor EL, a nonionic surfactant, at clinical concentrations significantly decreases the cell viability of rat thymocytes with phosphatidylserine-exposed (PS-exposed) membranes under in vitro condition. It is reminiscent of a possibility that sodium dodecylbenzenesulfonate (DCBS), an anionic surfactant world-widely used for detergents, also affects the cells in the similar manner. To test the possibility, the effect of DCBS on rat thymocytes has been ...
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Lidocaine is a local anesthetic possessing both lipophilic and hydrophilic properties. It also acts as a surfactant. Thus, the disruption of membranes, resulting in necrosis, is one of possible mechanisms for lidocaine-induced cytotoxicity. However, lidocaine is reported to induce apoptosis. Therefore, in order to compare two mechanisms for cell death induced by lidocaine, the effects of millimolar lidocaine were examined on rat thymocytes by a flow cytometer with appropriate fluorescent probes....
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Abstract Cremophor EL, a surfactant for pharmaceutical products, augments the cytotoxicity of hydrogen peroxide in rat thymocytes [Iwase, K., Oyama, Y., Tatsuishi, T., Yamaguchi1, J., Nishimura1, Y., Kanada, A., Kobayashi, M., Maemura, Y., Ishida, S., Okano, Y., 2004. Cremophor EL augments the cytotoxicity of hydrogen peroxide in lymphocytes dissociated from rat thymus glands. Toxicol. Lett. 154, 143–148]. The effect of cremophor EL on Ca 2+ -dependent process of cell death has been examined usi...
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