Towards a ‘universal’ nucleant for protein crystallization

Published on Feb 1, 2009in Trends in Biotechnology13.747
· DOI :10.1016/j.tibtech.2008.10.008
Emmanuel N. Saridakis52
Estimated H-index: 52
Naomi E. Chayen33
Estimated H-index: 33
(Imperial College London)
The expression ‘crystal clear' stems from the science of crystallography, which determines the detailed atomic structures of materials by exposing crystals to X-rays. Protein structures are pivotal to the success of rational drug design and other biotechnology applications; however, obtaining high quality crystals poses a major problem to progress. Nucleation is the first step that determines the entire crystallization process, thus control of crystal nucleation would tackle the problem at its conception. A search for a ‘universal' nucleant, a substance that can induce nucleation of any protein, is therefore on-going. We report the advances that have been made in this area, highlighting the success of especially engineered as well as naturally structured surfaces.
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