Fifty Years Excitement with Science: Recollections with and without tRNA

Published on Mar 1, 2013in Journal of Biological Chemistry
· DOI :10.1074/jbc.X113.453894
Richard Giegé54
Estimated H-index: 54
(UDS: University of Strasbourg)
  • References (48)
  • Citations (2)
#1Lisa G. Riley (Children's Hospital at Westmead)H-Index: 13
#2Sandra T. Cooper (USYD: University of Sydney)H-Index: 27
Last. John Christodoulou (USYD: University of Sydney)H-Index: 51
view all 12 authors...
Mitochondrial respiratory chain disorders are a heterogeneous group of disorders in which the underlying genetic defect is often unknown. We have identified a pathogenic mutation (c.156C>G [p.F52L]) in YARS2, located at chromosome 12p11.21, by using genome-wide SNP-based homozygosity analysis of a family with affected members displaying myopathy, lactic acidosis, and sideroblastic anemia (MLASA). We subsequently identified the same mutation in another unrelated MLASA patient. The YARS2 gene prod...
168 CitationsSource
#1Tania BourH-Index: 3
#2Aziza AkaddarH-Index: 2
Last. Magali FrugierH-Index: 23
view all 7 authors...
Distinctive features of aspartyl-transfer RNA (tRNA) synthetases (AspRS) from the protozoan Plasmodium genus are described. These apicomplexan AspRSs contain 29–31 amino acid insertions in their anticodon binding domains, a remarkably long N-terminal appendix that varies in size from 110 to 165 amino acids and two potential initiation codons. This article focuses on the atypical functional and structural properties of Plasmodium falciparum cytosolic AspRS, the causative parasite of human malaria...
24 CitationsSource
#1Kaouthar Dhouib (UDS: University of Strasbourg)H-Index: 3
#2Chantal Khan Malek (CNRS: Centre national de la recherche scientifique)H-Index: 11
Last. Claude Sauter (UDS: University of Strasbourg)H-Index: 22
view all 14 authors...
Microfluidic devices were designed to perform on micromoles of biological macromolecules and viruses the search and the optimization of crystallization conditions by counter-diffusion, as well as the on-chip analysis of crystals by X-ray diffraction. Chips composed of microchannels were fabricated in poly-dimethylsiloxane (PDMS), poly-methyl-methacrylate (PMMA) and cyclo-olefin-copolymer (COC) by three distinct methods, namely replica casting, laser ablation and hot embossing. The geometry of th...
75 CitationsSource
#1Sheng-Xiang LinH-Index: 24
#2Alexander McPhersonH-Index: 51
Last. Richard GiegéH-Index: 54
view all 3 authors...
The 11th International Conference on the Crystallization of Biological Macromolecules (ICCBM-11) organized by S.-X. Lin was held in Quebec City, Canada, Aug 16–21, 2006, supported by the International organization of Biological Crystallization and Laval University Medical Center. It was preceded by a Workshop on Advanced Crystallization Methods for Structural Biologists.
4 CitationsSource
#1Luc Bonnefond (CNRS: Centre national de la recherche scientifique)H-Index: 7
#2Magali Frugier (CNRS: Centre national de la recherche scientifique)H-Index: 23
Last. Joëlle Rudinger-Thirion (CNRS: Centre national de la recherche scientifique)H-Index: 18
view all 8 authors...
We report the structure of a strictly mitochondrial human synthetase, namely tyrosyl-tRNA synthetase (mt-TyrRS), in complex with an adenylate analog at 2.2 A resolution. The structure is that of an active enzyme deprived of the C-terminal S4-like domain and resembles eubacterial TyrRSs with a canonical tyrosine-binding pocket and adenylate-binding residues typical of class I synthetases. Two bulges at the enzyme surface, not seen in eubacterial TyrRSs, correspond to conserved sequences in mt-Tyr...
42 CitationsSource
#1J.-J. LeguayH-Index: 1
#2Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
Last. E. AnsoborloH-Index: 1
view all 4 authors...
3 CitationsSource
#1Michaël Ryckelynck (CNRS: Centre national de la recherche scientifique)H-Index: 5
#2Benoît Masquida (CNRS: Centre national de la recherche scientifique)H-Index: 18
Last. Magali Frugier (CNRS: Centre national de la recherche scientifique)H-Index: 23
view all 4 authors...
Accurate translation of genetic information necessitates the tuned expression of a large group of genes. Amongst them, controlled expression of the enzymes catalyzing the aminoacylation of tRNAs, the aminoacyl-tRNA synthetases, is essential to insure translational fidelity. In the yeast Saccharomyces cerevisiae, expression of aspartyl-tRNA synthetase (AspRS) is regulated in a process necessitating recognition of the 5′ extremity of AspRS messenger RNA (mRNAAspRS) by its translation product and a...
13 CitationsSource
#1Joseph D. Ng (UAH: University of Alabama in Huntsville)H-Index: 13
#2Claude Sauter (CNRS: Centre national de la recherche scientifique)H-Index: 22
Last. Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
view all 6 authors...
Protein crystallization under microgravity aims at benefiting from the quasi-absence of convection and sedimentation to favor well ordered crystal nucleation and growth. The dimeric multidomain enzyme aspartyl-tRNA synthetase from Thermus thermophilus has been crystallized within dialysis reactors of the Advanced Protein Crystallization Facility in the laboratory on earth and under microgravity aboard the US Space Shuttle. A strictly comparative crystallographic analysis reveals that the crystal...
22 CitationsSource
#1Richard GiegéH-Index: 54
#2Mark HelmH-Index: 39
Last. Catherine FlorentzH-Index: 43
view all 3 authors...
9 CitationsSource
#1Claude Sauter (CNRS: Centre national de la recherche scientifique)H-Index: 22
#2Bernard Lorber (CNRS: Centre national de la recherche scientifique)H-Index: 28
Last. Richard Giegé (CNRS: Centre national de la recherche scientifique)H-Index: 54
view all 5 authors...
Abstract Aminoacyl-tRNA synthetases catalyze the specific charging of amino acid residues on tRNAs. Accurate recognition of a tRNA by its synthetase is achieved through sequence and structural signalling. It has been shown that tRNAs undergo large conformational changes upon binding to enzymes, but little is known about the conformational rearrangements in tRNA-bound synthetases. To address this issue the crystal structure of the dimeric class II aspartyl-tRNA synthetase (AspRS) from yeast was s...
57 CitationsSource
Cited By2
#1Catherine Florentz (UDS: University of Strasbourg)H-Index: 43
#2Richard Giegé (UDS: University of Strasbourg)H-Index: 54
#2Wesley D. SwingleyH-Index: 13
Last. David H. Ardell (UCM: University of California, Merced)H-Index: 18
view all 3 authors...
Molecular phylogenetics and phylogenomics are subject to noise from horizontal gene transfer (HGT) and bias from convergence in macromolecular compositions. Extensive variation in size, structure and base composition of alphaproteobacterial genomes has complicated their phylogenomics, sparking controversy over the origins and closest relatives of the SAR11 strains. SAR11 are highly abundant, cosmopolitan aquatic Alphaproteobacteria with streamlined, A+T-biased genomes. A dominant view holds that...
7 CitationsSource