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Characterization of precrystallization aggregation of canavalin by dynamic light scattering.

Published on Jan 1, 1990in Biophysical Journal3.67
· DOI :10.1016/S0006-3495(90)82513-3
Webe Kadima4
Estimated H-index: 4
(UCR: University of California, Riverside),
Alexander McPherson53
Estimated H-index: 53
(UCR: University of California, Riverside)
+ 1 AuthorsFrances Jurnak25
Estimated H-index: 25
(UCR: University of California, Riverside)
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Abstract
The aggregation processes leading to crystallization and precipitation of canavalin have been investigated by dynamic light scattering (DLS) in photon correlation spectroscopy (PCS) mode. The sizes of aggregates formed under various conditions of pH, salt concentration, and protein concentrations were deduced from the correlation functions generated by the fluctuating intensity of light scattered by the solutions of the protein. Results obtained indicate that the barrier to crystallization of canavalin is the formation of the trimer, a species that has been characterized by x-ray crystallographic studies (McPherson, A. 1980. J. Biol. Chem. 255:10472–10480). The dimensions of the trimer in solution are in good agreement with those obtained both from the crystal (McPherson, A. 1980. J. Biol. Chem. 255:10472–10480) and from a low angle x-ray scattering study in solution (Plietz, P., P. Damaschun, J. J. Muller, and B. Schlener. 1983. FEBS [Fed. Eur. Biochem. Soc.] Lett. 162:43–46). Furthermore, under conditions known to lead to the formation of rhombohedral crystals of canavalin, a limiting size is reached at high concentrations of canavalin. The size measured corresponds to an aggregate of trimers making a unit rhombohedral cell consistent with x-ray crystallographic data (McPherson, A. 1980. J. Biol. Chem. 255:10472–10480). Presumably, such aggregates are the nuclei from which crystal growth proceeds. The present study was undertaken primarily to test the potential of DLS (PCS) as a tool for rapid, routine screening to determine the ultimate fate of protein solutions (i.e., crystallization or amorphous precipitation) at an early stage, therefore eliminating the need for long-term visual observation.(ABSTRACT TRUNCATED AT 250 WORDS)
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Tristan O. C. Kwan , Rosana Reis + 3 AuthorsIsabel Moraes9
Estimated H-index: 9
Over the years, there have been many developments and advances in the field of integral membrane protein research. As important pharmaceutical targets, it is paramount to understand the mechanisms of action that govern their structure–function relationships. However, the study of integral membrane proteins is still incredibly challenging, mostly due to their low expression and instability once extracted from the native biological membrane. Nevertheless, milligrams of pure, stable, and functional...
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Yue Yu2
Estimated H-index: 2
(JLU: Jilin University),
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Estimated H-index: 10
(JLU: Jilin University)
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Published on Jan 1, 2018in Nanoscale6.97
Mike Sleutel12
Estimated H-index: 12
(Vrije Universiteit Brussel),
Alexander E. S. Van Driessche1
Estimated H-index: 1
(IFSTTAR)
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Hilary P. Stevenson9
Estimated H-index: 9
(University of Pittsburgh),
Alexander M. Makhov17
Estimated H-index: 17
(University of Pittsburgh)
+ 13 AuthorsTed M. Ross22
Estimated H-index: 22
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Giuseppe Vitiello16
Estimated H-index: 16
(University of Naples Federico II),
Gaetano Mangiapia18
Estimated H-index: 18
(University of Naples Federico II)
+ 5 AuthorsGerardino D’Errico11
Estimated H-index: 11
(University of Naples Federico II)
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Richard Giegé53
Estimated H-index: 53
(CNRS: Centre national de la recherche scientifique)
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Published on Aug 12, 2013in ACS Combinatorial Science3.20
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Estimated H-index: 13
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Janet Newman26
Estimated H-index: 26
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Wei Wang1
Estimated H-index: 1
(SWU: Southwest University),
Chun Liu1
Estimated H-index: 1
(SWU: Southwest University)
+ 1 AuthorsChengZhi Huang5
Estimated H-index: 5
(SWU: Southwest University)
With the development of nanosciences, both localized surface plasmon resonance light scattering (LSPR-LS) and dynamic light scattering (DLS) techniques have been widely used for quantitative purposes with high sensitivity. In this contribution, we make a comparison of the two light scattering techniques by employing gold nanoparticles (AuNPs) aggregation induced by mercuric ions. It was found that citrate-stabilized AuNPs got aggregated in aqueous medium in the presence of mercuric ions through ...
Published on Sep 20, 2012
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