A rapid and sensitive reporter gene that uses green fluorescent protein expression to detect chemicals with estrogenic activity.
Abstract
A reporter gene sequence was constructed within a eukaryotic expression vector. The altered plasmid contained 2 sequential estrogen response elements (ERE) coupled to a human phosphoglycerate kinase (PGK) promoter inserted upstream from a cDNA sequence encoding enhanced green fluorescent protein (GFP) with a 3'-polyadenylation signal. The plasmid was linearized and transfected into MCF-7 cells, a human breast cancer-derived line that expresses...
Paper Details
Title
A rapid and sensitive reporter gene that uses green fluorescent protein expression to detect chemicals with estrogenic activity.
Published Date
May 1, 2000
Journal
Volume
55
Issue
1
Pages
69 - 77
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