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Electrostatic interaction of poly(L-lysine) with dipalmitoylphosphatidic acid studied by X-ray diffraction.

Published on Nov 1, 1991in Biochimica et Biophysica Acta3.79
· DOI :10.1016/0005-2736(91)90129-V
Hideyuki Takahashi68
Estimated H-index: 68
(Nagoya University),
Sinzi Matuoka6
Estimated H-index: 6
(Nagoya University)
+ 2 AuthorsIchiro Hatta16
Estimated H-index: 16
(Nagoya University)
Abstract
Abstract Structure of dipalmitoylphosphatidic acid (DPPA) bilayers in the presence of poly( l -lysine) is proposed from the results of X-ray diffraction obtained by a storage phosphir detector with a high resolution called an imaging plate. The small-angle X-ray diffraction pattern exhibits that DPPA/poly( l -lysine) complex forms a highly ordered multilamellar structure. The electron density profile of the DPPA/poly( l -lysine) complex draws that only one poly( l -lysine) layer is intercalated betweenthe neighboring DPPA bilayers. The wide-angle X-ray diffraction pattern suggests that the presence of poly( l -lysine) hardly affects the nature of hydrocarbon chain packing in the DPPA bilayers. The X-ray reflection from the DPPA/poly( l -lysine) complex indicates that the poly( l -kysine) molecules adopt a β-sheet conformation on the surface of the DPPA bilayers. The both surface areas occupied by a headgroup of the DPPA and by a lysine residue in poly( l -lysine) are estimated from the observed spacings. The number ratio of lysine residues to DPPA headgroups per unit area is greaterthan unity. Therefore, one DPPA headgroup interacts with more than one lysine residue electrostatically, i.e., the electric charge distributions in both the surface of a DPPA bilayer and the poly( l -lysine) β-sheet are incommensurate.
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