A simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose

Published on Oct 1, 2009in Journal of Applied Crystallography2.867
· DOI :10.1107/S0021889809028805
Tae Woong Whon1
Estimated H-index: 1
(Korea Research Institute of Bioscience and Biotechnology),
Yong Hwan Lee1
Estimated H-index: 1
(LSU: Louisiana State University)
+ 2 AuthorsSong G. Kim1
Estimated H-index: 1
(Korea Research Institute of Bioscience and Biotechnology)
A simple protocol to convert sitting-drop vapor-diffusion plating into a hanging-drop vapor-diffusion experiment in protein crystallization is reported. After making a sitting-drop plate, agarose solution was added to solidify the reservoir solution, and the plates were incubated upside down. Crystallization experiments with hen egg white lysozyme, thaumatin and glucose isomerase showed that the `upside-down sitting-drop' method could produce single crystals with all the benefits of the hanging-drop crystallization method.
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