Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

Nature64.80
Volume: 513, Issue: 7519, Pages: 569 - 573
Published: Sep 25, 2014
Abstract
The CRISPR-associated protein Cas9 is an RNA-guided endonuclease that cleaves double-stranded DNA bearing sequences complementary to a 20-nucleotide segment in the guide RNA. Cas9 has emerged as a versatile molecular tool for genome editing and gene expression control. RNA-guided DNA recognition and cleavage strictly require the presence of a protospacer adjacent motif (PAM) in the target DNA. Here we report a crystal structure of Streptococcus...
Paper Details
Title
Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
Published Date
Sep 25, 2014
Journal
Volume
513
Issue
7519
Pages
569 - 573
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