Determination of carbonyl compounds by the oxidative chemiluminescence of 2,4-dinitrophenylhydrazine

The oxidative chemiluminescence of phenylhydrazines can be applied to the determination of carbonyl compounds by utilising the attenuation of the signal which occurs on conversion to the corresponding phenylhydrazone. Using an assay for 2,4-dinitrophenylhydrazine, off-line derivatisation for a period of 2 h was found to be optimised for a temperature of 30 °C and for a sulfuric acid concentration of 0.05 M. The optimum conditions for...