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H2O2 induced lipid peroxidation in rat brain homogenates is greatly reduced by melatonin

Published on Aug 1, 1995in Neuroscience Letters2.173
· DOI :10.1016/0304-3940(95)11803-5
Ewa Sewerynek21
Estimated H-index: 21
(University of Texas Health Science Center at San Antonio),
Burkhard Poeggeler36
Estimated H-index: 36
(University of Texas Health Science Center at San Antonio)
+ 1 AuthorsRussel J. Reiter149
Estimated H-index: 149
(University of Texas Health Science Center at San Antonio)
Sources
Abstract
Abstract Melatonin protection against H 2 O 2 -induced lipid peroxidation in brain homogenates was measured in vitro. The level of malonaldehyde (MDA) plus 4-hydroxyalkenals (4-HDA) was assayed in brain homogenates as an index of induced membrane oxidative damage. Brain homogenates were co-incubated with H 2 O 2 alone or in combination with melatonin. Brain MDA + 4-HDA increased after H202 (0.1–5 mM) with the effect being both concentration- and time-dependent. Melatonin (0.1–5 mM) protected against H 2 O 2 -induced lipid peroxidation of brain homogenates in a concentration-dependent manner.
  • References (15)
  • Citations (55)
References15
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#1Ewa Sewerynek (University of Texas Health Science Center at San Antonio)H-Index: 7
#2Mitsushi Abe (University of Texas Health Science Center at San Antonio)H-Index: 6
Last. Beatriz Diaz‐Lopez (University of Texas Health Science Center at San Antonio)H-Index: 1
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The protective effect of melatonin on lipopolysaccharide (LPS)-induced oxidative damage in phenobarbital-treated rats was measured using the following parameters: changes in total glutathione (tGSH) concentration, levels of oxidized glutathione (GSSG), the activity of the antioxidant enzyme glutathione peroxidase (GSH-PX) in both brain and liver, and the content of cytochrome P450 reductase in liver. Melatonin was injected intraperitoneally (ip, 4mg/kg BW) every hour for 4 h after LPS administra...
109 CitationsSource
#1Lornell Barlow-Walden (University of Texas Health Science Center at San Antonio)H-Index: 10
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Abstract Exogenously administered melatonin causes a 2-fold rise in glutathione peroxidase activity within 30 min in the brain of the rat. Furthermore, brain glutathione peroxidase activity is higher at night than during the day and is correlated with high night-time tissue melatonin levels. Glutathione peroxidase is thought to be the principal enzyme eliminating peroxides in the brain. This antioxidative enzyme reduces the formation of hydroxyl radicals formed via iron-catalyzed Fenton-type rea...
479 CitationsSource
#1Russel J. Reiter (University of Texas Health Science Center at San Antonio)H-Index: 149
The debilitating consequences of age-related brain deterioration are widespread and extremely costly in terms of quality of life and longevity. One of the potential major causes of age-related destruction of neuronal tissue is toxic free radicals that are a natural result of aerobic metabolism. The brain is particularly susceptible to free radical attack because it generates more of these toxicants per gram of tissue than does any other organ. The major defense mechanisms the brain uses to comba...
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#2Daniela Melchiorri (University of Texas Health Science Center at San Antonio)H-Index: 18
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This survey summarizes the findings, accumulated within the last 2 years, concerning melatonin's role in defending against toxic free radicals. Free radicals are chemical constituents that have an unpaired electron in their outer orbital and, because of this feature, are highly reactive. Inspired oxygen, which sustains life, also is harmful because up to 5% of the oxygen (O 2 ) taken in is converted to oxygen-free radicals. The addition of a single electron to O 2 produces the superoxide anion r...
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Abstract The in vivo effect of melatonin on paraquat-induced oxidative damage in rat lung and liver was studied using two parameters: the concentration of malonaldehyde and 4-hydroxyalkenals as indices of lipid peroxidation; changes in total and oxidized glutathione. Melatonin (10 mg/kg) or an equal volume of saline were administered intraperitoneally (ip) to rats 30 min prior to an ip injection of paraquat (20mg/kg or 70mg/kg). After paraquat treatment, the animals received melatonin or saline ...
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Abstract We have compared the peroxyl radical scavenger ability of melatonin with that of vitamin E, vitamin C and reduced glutathione (GSH). In the assay system, β-phycoerythrin (β-PE) was used as fluorescent indicator protein, 2-2′-azo-bis(2-amidinopropane)dihydrochloride as a peroxyl radical generator and the water soluble vitamin E analogue, Trolox, as reference standard. Results are expressed as oxygen radical absorbing capacity (ORAC perox ) units, where 1 ORAC unit equals the net protecti...
627 CitationsSource
#1Irina I. Karuzina (Academy of Medical Sciences, United Kingdom)H-Index: 14
#2Alexander I. Archakov (Academy of Medical Sciences, United Kingdom)H-Index: 32
Possible mechanisms of cytochrome P450 self-inactivation during catalytic turnover have been considered. Two ways of hemoprotein inactivation are so far known. The firts, studied extensively by many authors, is the formation of active substrate intermediates, capable of modifying heme and apoenzyme. The second way, revealed quite recently and resulting from uncoupled cytochrome P450-catalyzed monooxygenase reactions, is yet to be clarified. Briefly, it involves formation of hydrogen peroxide in ...
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Pages 139–162: Yu. “Cellular Defenses Against Damage From Reactive Oxygen Species.” Page 150: The section 2. Ceruloplasmin should appear as follows: [See PDF]
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#1Armando Menendez-Pelaez (University of Texas Health Science Center at San Antonio)H-Index: 22
#2Burkhard Poeggeler (University of Texas Health Science Center at San Antonio)H-Index: 36
Last. Dun Xian Tan (University of Texas Health Science Center at San Antonio)H-Index: 97
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Melatonin was detected by an improved immunocytochemical technique in the cell nuclei of most tissues studied including several brain areas, pineal gland, Harderian gland, gut, liver, kidney, and spleen from rodents and primates. Cryostat sections from tissues fixed in Bouin's fluid, formalin, or acetone/ethanol were used. The nuclear staining appeared primarily associated with the chromatin. The nucleoli did not exhibit a positive reaction. The melatonin antiserum was used in the range of 1:500...
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#1Burkhard Poeggeler (University of Texas Health Science Center at San Antonio)H-Index: 36
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Abstract: Melatonin is a very potent and efficient endogenous radical scavenger. The pineal indolamine reacts with the highly toxic hydroxyl radical and provides on-site protection against oxidative damage to biomolecules within every cellular compartment. Melatonin acts as a primary non-enzymatic antioxidative defense against the devastating actions of the extremely reactive hydroxyl radical. Melatonin and structurally related tryptophan metabolites are evolutionary conservative molecules princ...
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