Kinetic Modeling at Single-Molecule Resolution Elucidates the Mechanisms of Cellulase Synergy

The mechanism of synergistic cooperation between cellulases in decomposing cellulose is revealed by resolving the molecular structures of enzymes and substrates via kinetic modeling. The emergence of endo–exo and exo–exo synergy through enzyme–enzyme and enzyme–substrate couplings was investigated with the main exoglucanases (TrCel7A and TrCel6A) and endoglucanase (TrCel7B) of the Trichoderma reesei fungus. The degree of synergy was found to...