Gradient reversed-phase high-performance chromatography of ionogenic analytes
Volume: 30, Issue: 9, Pages: 1372 - 1381
Published: Oct 1, 2011
Abstract
This review highlights the basis of gradient reversed-phase high-performance liquid chromatography (RP-HPLC) of ionogenic analytes. We describe pH-gradient RP-HPLC in strict theoretical terms, with examples of experiments to provide improvements in analyte separations and peak shapes, and an original method of pKa determination. Finally, we present the concept of the combined pH/organic-modifier gradient mode of RP-HPLC and illustrate it with...
Paper Details
Title
Gradient reversed-phase high-performance chromatography of ionogenic analytes
Published Date
Oct 1, 2011
Volume
30
Issue
9
Pages
1372 - 1381
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