Match!

Sterol composition of gonad, muscle and digestive gland of Pecten maximus from Málaga (South Spain)

Published on Mar 1, 2003in Comparative Biochemistry and Physiology B1.99
· DOI :10.1016/S1096-4959(02)00284-1
Antonio J. Pazos14
Estimated H-index: 14
(University of Santiago de Compostela),
Arturo Silva5
Estimated H-index: 5
(University of Santiago de Compostela)
+ 4 AuthorsM. Abad14
Estimated H-index: 14
(University of Santiago de Compostela)
Abstract
Sterol composition and content and their seasonal variations over 18 months were investigated in adductor muscle, digestive gland and gonads of Pecten maximus. Sterols were isolated by Silicagel 60 thin layer chromatography and identified by gas chromatography/mass spectrometry. Eleven sterols were identified, with cholesterol, brassicasterol, 24-methylenecholesterol and 22-trans-dehydrocholesterol being the principal components. The same sterols were found in all three tissues independent of season. The relative amounts of each sterol present in each tissue differed. Total sterol levels in gonad and muscle were higher than in digestive gland. Statistically significant differences (P<0.05) were found between the concentrations of each of the sterols isolated from the gonad or muscle and digestive gland. The seasonal variations in the sterol content of the gonad seem be related to the reproductive cycle, while the sterol content of the digestive gland appears to be linked to diet, mainly diatoms or dinoflagellates. The muscle sterol content showed minor changes throughout the year.
  • References (28)
  • Citations (12)
📖 Papers frequently viewed together
1982
1 Author (B. Blanchier)
29 Citations
109 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
References28
Newest
Abstract The assemblages of diatoms contained in nineteen surface sediment samples recovered off the coast of Malaga province (Spain) between 5°W and 4°38′W have been analysed. The variations observed in the diatom assemblage point to the presence of nutrient-enriched waters, transported laterally from the centre of an upwelling that occurs to the west of the study area. The utility of species such as Paralia sulcata, Thalassionema nitzschioides and the spores of Chaetoceros spp. in the reconstr...
48 CitationsSource
#1Philippe Soudant ('IFREMER': IFREMER)H-Index: 44
#2Jean-Rene Le Coz ('IFREMER': IFREMER)H-Index: 8
Last. Jean-Francois Samain ('IFREMER': IFREMER)H-Index: 32
view all 6 authors...
Changes in sterol composition of Pecten maximus larvae during the larval development stage with standard algal mixtures and unialgal diets were analysed. The sterol composition of four microalgae currently used in mollusc hatchery were also examined. Under standard algal conditions, the larvae quickly use the steryl ester from larvae reserves during the endotrophic and the mixotrophe phases. The preferential incorporation of Pavlova lutheri and T-Isochrysis sterols, rather than Skeletonema costa...
52 CitationsSource
#1Antonio J. Pazos (University of Santiago de Compostela)H-Index: 14
#2Guillermo RománH-Index: 9
Last. M. Abad (University of Santiago de Compostela)H-Index: 14
view all 5 authors...
63 CitationsSource
#1Philippe Soudant ('IFREMER': IFREMER)H-Index: 44
#2Yanic Marty (CNRS: Centre national de la recherche scientifique)H-Index: 25
Last. Jean François Samain ('IFREMER': IFREMER)H-Index: 7
view all 7 authors...
Abstract Spawning individuals of the scallop Pecten maximus were conditioned on three test diets: Tahiti Isochrysis , a mixture (PTSC) and Chaetoceros calcitrans . The scallops fed T- Isochrysis showed a better hatching rate and lower atresia than those fed the other two diets. Proximate composition of the female gonads was not modified by the differences in the diets. Enrichment of gonads with sterol esters and triglycerides, characteristic of storage of lipids, observed with the broodstock fed...
145 CitationsSource
#1Valeriano Rodríguez (UMA: University of Málaga)H-Index: 16
#2Francisco José Guerrero Ruiz (UMA: University of Málaga)H-Index: 6
The chlorophyll a content of four different size fractions (<2, 2-20, 20-45, 45-100 μm) of phytoplankton at a coastal station of Malaga Bay (South Spain) were studied throughout the summer and autumn of 1989. Pigment concentrations in the surface of sediments were also measured. Episodes of increased chlorophyll a concentration revealed the transitory existence of several blooms. The large increases in biomass consisted mostly of nanoplankton (2-20 μm) and this generally represents some 50% of t...
9 CitationsSource
#1Fidel EchevarríaH-Index: 23
#2Begoña Bautista (UMA: University of Málaga)H-Index: 9
Last. Valeriano Rodríguez (UMA: University of Málaga)H-Index: 16
view all 4 authors...
4 CitationsSource
#1G. E. Napolitano (Dal: Dalhousie University)H-Index: 3
#2R. G. Ackman (Technical University of Nova Scotia)H-Index: 5
Last. M. A. Silva-Serra (Dal: Dalhousie University)H-Index: 1
view all 3 authors...
40 CitationsSource
#1Guillermo E. Napolitano (Dal: Dalhousie University)H-Index: 4
#2Robert G. Ackman (Technical University of Nova Scotia)H-Index: 34
Abstract 1. 1. Lipid contents, lipid class compositions and their temporal variations were studied in the sea scallop Placopecten magellanicus (Gmelin) from Georges Bank. 2. 2. Analyses of the major organs (digestive gland, gonad, adductor muscle, mantle, and gills) presented different and characteristic lipid profiles. 3. 3. The lipid contents of adductor muscle, gills, mantle and male gonad usually ranged from 0.6 to 1% of the wet weight. Lipids in these organs were mostly structural phospholi...
39 CitationsSource
#1Glenn W. PattersonH-Index: 1
166 Citations
#1Glenn PattersonH-Index: 1
58 CitationsSource
Cited By12
Newest
#1Bernhard KleineH-Index: 6
During the evolution of life, singled-celled organisms developed into multicellular ones and these multicellular organisms became plants or animals. When animals got organs with diverse functions, they had to invent communication between these to establish homeostasis. The devices of this communication are nerves and hormones.
Source
#1E. Perrat (University of Nantes)H-Index: 1
#1E. Perrat (University of Nantes)H-Index: 3
Last. G. Wielgosz-Collin (University of Nantes)H-Index: 1
view all 5 authors...
Abstract Lipids are central for energy metabolism and their fate in bivalves is closely linked to environmental conditions and gametogenic cycle. In order to assess the pollution impact on lipid metabolism of bivalves, storage and structure lipids from samples of Scrobicularia plana were studied. These samples were collected during sexual maturity both from estuaries considered contaminated (Goyen and Blavet) and from a reference site (Bay of St Brieuc) for comparison. Lipids were extracted from...
21 CitationsSource
#1A. V. V. M SriH-Index: 1
Comprehensive inventory of the bioactive compounds produced by Anadara granosaduring full growth has been established by whole tissue using organic solvents system and subjecting in to gas chromatograp hy mass spectrum (GC-MS) twelve bioactive compounds of which ester, furan, acid and ketone were identified. The most representative bioactive compounds of Anadara granosawere employed in the antibacterial activity against pathogenic bacteria and the study pertaining to the identification of active...
7 Citations
#1Renée Gagné (Université du Québec à Rimouski)H-Index: 2
#2Réjean Tremblay (Université du Québec à Rimouski)H-Index: 27
Last. Frédéric Olivier (CNRS: Centre national de la recherche scientifique)H-Index: 21
view all 6 authors...
The main objective of this study was to evaluate the effect of the addition of Rhodomonas salina in the diet of Pecten maximus on growth, survival and metamorphosis success in relation to biochemical content. Food quality is an essential factor in the success of larval, post-larval and juvenile development. A diet rich in polyunsaturated fatty acids present an advantage for growth, survival and metamorphosis success. Larvae and post-larvae fed with the diet containing R. salina showed a higher a...
17 CitationsSource
#1Wenbing Zhang (Ocean University of China)H-Index: 30
#2Kangsen Mai (Ocean University of China)H-Index: 47
Last. Xiaojie Wang (Ocean University of China)H-Index: 19
view all 7 authors...
Abstract An experiment was conducted to determine the dietary cholesterol requirement of juvenile abalone Haliotis discus hannai . Eight isoenergetic (18.15–18.61 kJ/g) and isonitrogenous (29.00–29.78% protein) diets, supplemented with 0.00, 0.15, 0.30, 0.45, 0.60, 0.75, 0.90 and 1.05% cholesterol were evaluated. Juvenile abalone (initial weight: 0.67–0.72 g) were reared in a flow-through water system for 24 weeks. During the feeding trial, water temperature was maintained at 14–17 °C, salinity ...
5 CitationsSource
#1Qiujin Zhu (Guida: Guizhou University)H-Index: 1
#2Li-Ping Wang (Jiangnan University)H-Index: 2
Last. Jian Tang (Jiangnan University)H-Index: 5
view all 6 authors...
Abstract MIPs (molecularly imprinted polymers) for 17β-estradiol were prepared using 2-(trifluoromethyl) acrylic acid (TFMAA) as functional monomer and trimethylolpropane trimethacrylate (TRIM) as cross-linker by precipitation polymerisation. Data from HPLC system were calculated, and separation properties of several MIPs were reported. MISPEs (molecularly imprinted solid phase extractions) were performed with MIPs as sorbent, and absorption properties of the MISPEs prepared by UV irradiation in...
26 CitationsSource
#1A. N. A M. A. T. Us. De La Parra (University of Vigo)H-Index: 1
#2Oscar García (University of Santiago de Compostela)H-Index: 15
Last. F. U. E. N. C. Isla. San Juan (University of Vigo)H-Index: 1
view all 3 authors...
Abstract Variations in biochemical composition and lipid classes in relations to the gametogenic cycle were studied in digestive gland, labial palps and gonad in the Pacific oyster Crassostrea gigas from the Western Atlantic coast of France over 1 y. Variations in the weight of the soft body parts, gonad development and spawning depended on environmental conditions, mainly on nutrients availability. The main energy reserve is the glycogen, showing a marked seasonal variability in all tissues stu...
22 CitationsSource
#1Ana Alonso Martinez (University of Vigo)H-Index: 2
#2Yolanda Ruiz Muñoz (University of Vigo)H-Index: 1
Last. Pilar Molist García (University of Vigo)H-Index: 2
view all 4 authors...
We have examined the localization of P450scc in different tissues of the mollusc Mytilus galloprovincialis along a gonadal cycle by using a polyclonal antibody against rat cholesterol side-chain cleavage enzyme (cytochrome P450scc). Immunoreactivity specific for P450scc was found only in the cytoplasm of basophilic cells from digestive gland; gonad, gills and kidney appeared to be devoid of P450scc immunoreactivity. SDS-gel electrophoresis and western blot analysis of different subcellular fract...
7 CitationsSource
#1Qiujin Zhu (Yangtze University)H-Index: 1
#2Jian Tang (Yangtze University)H-Index: 2
Last. Shangwei Chen (Yangtze University)H-Index: 3
view all 5 authors...
Submicron-scaled molecularly imprinted polymer (MIP) particles for 17-β-estradiol were prepared by precipitation polymerization method using 2-(trifluoromethyl) acrylic acid as functional monomer and trimethylolpropane trimethacrylate as cross-linker in acetonitrile. The obtained submicron-scaled MIP particles were subsequently packed into stainless steel columns and the chromatographic characteristics of the MIP stationary phase were investigated. Interestingly, it was found that tandem MIP col...
16 CitationsSource
#1Hideki Kawashima (Iwate Prefectural University)H-Index: 8
#2Masao Ohnishi (Obihiro University of Agriculture and Veterinary Medicine)H-Index: 26
Last. Mikio Kinoshita (Obihiro University of Agriculture and Veterinary Medicine)H-Index: 17
view all 5 authors...
Sterol composition in muscle and viscera tissues of the marine bivalve Megangulus zyonoensis was investigated. Among 13 different sterols identified by gas chromatography-mass spectrometry of their trimethylsilyl ether derivatives, cholesterol was the most abundant sterol, followed by 24-methylenecholesterol, in both muscle and viscera tissues. In this study, the proportion of brassicasterol, in addition to that of cholesterol, differed between the muscle and viscera.
9 CitationsSource