The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions

Gene3.50
Volume: 71, Issue: 2, Pages: 491 - 499
Published: Nov 1, 1988
Abstract
Polymerase chain reaction conditions were established for the in vitro amplification of eukaryotic small subunit ribosomal (16S-like) rRNA genes. Coding regions from algae, fungi, and protozoa were amplified from nanogram quantities of genomic DNA or recombinant plasmids containing rDNA genes. Oligodeoxynucleotides that are complementary to conserved regions at the 5' and 3' termini of eukaryotic 16S-like rRNAs were used to prime DNA synthesis...
Paper Details
Title
The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions
Published Date
Nov 1, 1988
Journal
Volume
71
Issue
2
Pages
491 - 499
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