A novel antilithiatic protein from Tribulus terrestris having cytoprotective potency.

Published on Jul 31, 2012in Protein and Peptide Letters1.168
· DOI :10.2174/092986612801619552
Archana Aggarwal6
Estimated H-index: 6
(JUIT: Jaypee University of Information Technology),
Simran Tandon8
Estimated H-index: 8
+ 1 AuthorsChanderdeep Tandon14
Estimated H-index: 14
Adhesion of calcium oxalate (CaOx) crystals to kidney cells is a key event in kidney stones associated with marked hyperoxaluria. As the propensity of stone recurrence and persistent side effects are not altered by surgical techniques available, phytotherapeutic agents could be useful as an adjuvant therapy. The present study is aimed at examining the antilithiatic potency of the protein biomolecules of Tribulus terrestris, a plant which is a common constituent of herbal marketed preparations to treat urolithiasis. Various biochemical methods with mass spectrometry were used to purify and characterize the purified protein. The protective potency of the protein was tested on the oxalate induced injury on renal epithelial cell lines (NRK 52E). An antilithiatic protein having molecular weight of ~ 60kDa was purified. This purified protein showed similarities with Carotenoid cleavage dioxygenase 7 (CCD7) of Arabidopsis thaliana after matching peptide mass fingerprints in MASCOT search engine. An EF hand domain was identified in CCD7 by SCAN PROSITE. Presence of an EF hand domain, a characteristic feature of calcium binding proteins and a role in the synthesis of retinol which is transported by retinol binding protein, a protein found in kidney stone matrix; of CCD7 support the role of TTP as an antilithiatic protein. The protective potency of TTP on NRK 52E was quite comparable to the aqueous extract of cystone. Our findings suggest that this purified protein biomolecule from Tribulus terrestris could open new vista in medical management of urolithiasis.
  • References (0)
  • Citations (20)
📖 Papers frequently viewed together
53 Citations
75 Citations
37 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
Cited By20
An annual herb, found in India, Iran, Mediterranean region, China, Black Sea, Australia, Africa, and generally in all tropical countries. The plant was known to Dioscorides and Pliny, as both mentioned it in their writings. Dioscorides and Galen stated that it increases semen, relieves dysuria and urinary bladder pain, and disintegrates both kidney and bladder stones. Hindus in India medicinally use both the fruit and the root, as they regard them having cooling, diuretic, tonic and aphrodisiac ...
#2Marziyeh Hajialyani (Kermanshah University of Medical Sciences)H-Index: 6
Last. Anupam Bishayee (UM: University of Miami)H-Index: 45
view all 7 authors...
Kidney stones are one of the oldest known and common diseases in the urinary tract system. Various human studies have suggested that diets with a higher intake of vegetables and fruits play a role in the prevention of kidney stones. In this review, we have provided an overview of these dietary plants, their main chemical constituents, and their possible mechanisms of action. Camellia sinensis (green tea), Rubus idaeus (raspberry), Rubia cordifolia (common madder), Petroselinum crispum (parsley),...
19 CitationsSource
#1Vijay V. Wagh (NBRI: National Botanical Research Institute)H-Index: 5
#2Ashok K. Jain (JU: Jiwaji University)H-Index: 7
Tribal community of Jhabua district uses the forest resources especially plants primarily for curing various ailments. Ethnobotanical practice has prevailed in this area since the ancient time and the invasive plants are not the exception. Thus, we documented the medicinal uses of the invasive plants. Exhaustive field surveys were conducted during 2008–2013 for the collection of the ethnobotanical data and voucher specimen. Information regarding ethnobotanical uses of plants was collected from t...
6 CitationsSource
#1Jyoti Kaushik (Amity Institute of Biotechnology)H-Index: 1
#2Simran Tandon (Amity University)H-Index: 8
Last. Chanderdeep Tandon (Amity Institute of Biotechnology)H-Index: 14
view all 6 authors...
: Tribulus terrestris has significant antilithiatic efficacy established via both in vitro as well as in vivo studies and is used in numerous anti-urolithiatic herbal formulations viz. Cystone, Uriflow, Uritone and Neeri. However, to fully utilize its antilithiatic potential, the influence of different extraction parameters on antilithiatic ability of T. terrestris aqueous extract needs elucidation. Thus, the current study was undertaken using statistically optimized extraction conditions for aq...
4 CitationsSource
Preclinical Research Miniature proteins are a class of oligopeptide characterized by their short sequence lengths and ability to adopt well-folded, three-dimensional structures. Because of their biomimetic nature and synthetic tractability, miniature proteins have been used to study a range of biochemical processes including fast protein folding, signal transduction, catalysis and molecular transport. Recently, miniature proteins have been gaining traction as potential therapeutic agents because...
2 CitationsSource
#1Wenyi Zhu (BTBU: Beijing Technology and Business University)H-Index: 1
#2Yijie Du (BTBU: Beijing Technology and Business University)H-Index: 1
Last. Li Li (BTBU: Beijing Technology and Business University)H-Index: 5
view all 5 authors...
Tribulus terrestris L. (TT) is an annual plant of the family Zygophyllaceae that has been used for generations to energize, vitalize, and improve sexual function and physical performance in men. The fruits and roots of TT have been used as a folk medicine for thousands of years in China, India, Sudan, and Pakistan. Numerous bioactive phytochemicals, such as saponins and flavonoids, have been isolated and identified from TT that are responsible alone or in combination for various pharmacological ...
12 CitationsSource
#1Deepak M. Kasote (Aberd.: University of Aberdeen)H-Index: 10
#2Suresh Jagtap (Bharati Vidyapeeth University)H-Index: 9
Last. Wendy R. Russell (Aberd.: University of Aberdeen)H-Index: 25
view all 5 authors...
Abstract Ethanopharmacological relevance The process of formation or appearance of a urinary stone anywhere in the renal tract is known as urolithiasis. It is a longstanding health problem, known to exist since early age of civilization. Records about symptoms, signs and treatment strategies of urinary stones diseases are found in the several ancient texts of traditional medicines such as Ayurveda, Traditional Chinese Medicine (TCM), Siddha and Unani. In Ayurveda, urolithiasis has been considere...
16 CitationsSource
#1Ikshit Sharma (Hamdard University)H-Index: 1
#2Washim Khan (Hamdard University)H-Index: 2
Last. Sayeed Ahmad (Hamdard University)H-Index: 16
view all 3 authors...
AbstractContext: Tribulus terrestris L. (Zygophyllaceae) fruits have long been used in traditional systems of medicine for the treatment of various urinary diseases including urolithiasis.Objective: To explore the anti-urolithiatic potential of gokhru and to develop an analytical method for quantitative estimation of metabolites for its quality control.Materials and methods: Aqueous extract of gokhru fruit was prepared through maceration followed by decoction to produce a mother extract, which w...
6 CitationsSource