A novel antilithiatic protein from Tribulus terrestris having cytoprotective potency.

Published on Jul 1, 2012in Protein and Peptide Letters1.17
· DOI :10.2174/092986612801619552
Anshu Aggarwal5
Estimated H-index: 5
Simran Tandon7
Estimated H-index: 7
+ 1 AuthorsChanderdeep Tandon14
Estimated H-index: 14
Adhesion of calcium oxalate (CaOx) crystals to kidney cells is a key event in kidney stones associated with marked hyperoxaluria. As the propensity of stone recurrence and persistent side effects are not altered by surgical techniques available, phytotherapeutic agents could be useful as an adjuvant therapy. The present study is aimed at examining the antilithiatic potency of the protein biomolecules of Tribulus terrestris, a plant which is a common constituent of herbal marketed preparations to treat urolithiasis. Various biochemical methods with mass spectrometry were used to purify and characterize the purified protein. The protective potency of the protein was tested on the oxalate induced injury on renal epithelial cell lines (NRK 52E). An antilithiatic protein having molecular weight of ~ 60kDa was purified. This purified protein showed similarities with Carotenoid cleavage dioxygenase 7 (CCD7) of Arabidopsis thaliana after matching peptide mass fingerprints in MASCOT search engine. An EF hand domain was identified in CCD7 by SCAN PROSITE. Presence of an EF hand domain, a characteristic feature of calcium binding proteins and a role in the synthesis of retinol which is transported by retinol binding protein, a protein found in kidney stone matrix; of CCD7 support the role of TTP as an antilithiatic protein. The protective potency of TTP on NRK 52E was quite comparable to the aqueous extract of cystone. Our findings suggest that this purified protein biomolecule from Tribulus terrestris could open new vista in medical management of urolithiasis.
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Cited By18
Published on Mar 7, 2018in International Journal of Molecular Sciences4.18
Mina Nirumand1
Estimated H-index: 1
Marziyeh Hajialyani2
Estimated H-index: 2
+ 4 AuthorsAnupam Bishayee39
Estimated H-index: 39
Kidney stones are one of the oldest known and common diseases in the urinary tract system. Various human studies have suggested that diets with a higher intake of vegetables and fruits play a role in the prevention of kidney stones. In this review, we have provided an overview of these dietary plants, their main chemical constituents, and their possible mechanisms of action. Camellia sinensis (green tea), Rubus idaeus (raspberry), Rubia cordifolia (common madder), Petroselinum crispum (parsley),...
Published on Jan 1, 2018in South African Journal of Botany1.50
Vijay V. Wagh4
Estimated H-index: 4
(NBRI: National Botanical Research Institute),
Ashok K. Jain5
Estimated H-index: 5
(JU: Jiwaji University)
Tribal community of Jhabua district uses the forest resources especially plants primarily for curing various ailments. Ethnobotanical practice has prevailed in this area since the ancient time and the invasive plants are not the exception. Thus, we documented the medicinal uses of the invasive plants. Exhaustive field surveys were conducted during 2008–2013 for the collection of the ethnobotanical data and voucher specimen. Information regarding ethnobotanical uses of plants was collected from t...
Published on Dec 1, 2017in Chemistry Central Journal2.09
Wenyi Zhu1
Estimated H-index: 1
(BTBU: Beijing Technology and Business University),
Yijie Du1
Estimated H-index: 1
(BTBU: Beijing Technology and Business University)
+ 2 AuthorsLi Li4
Estimated H-index: 4
(BTBU: Beijing Technology and Business University)
Tribulus terrestris L. (TT) is an annual plant of the family Zygophyllaceae that has been used for generations to energize, vitalize, and improve sexual function and physical performance in men. The fruits and roots of TT have been used as a folk medicine for thousands of years in China, India, Sudan, and Pakistan. Numerous bioactive phytochemicals, such as saponins and flavonoids, have been isolated and identified from TT that are responsible alone or in combination for various pharmacological ...
Published on Sep 1, 2017in Drug Development Research1.74
Justin M. Holub12
Estimated H-index: 12
(OU: Ohio University)
Preclinical Research Miniature proteins are a class of oligopeptide characterized by their short sequence lengths and ability to adopt well-folded, three-dimensional structures. Because of their biomimetic nature and synthetic tractability, miniature proteins have been used to study a range of biochemical processes including fast protein folding, signal transduction, catalysis and molecular transport. Recently, miniature proteins have been gaining traction as potential therapeutic agents because...
Published on May 1, 2017in Journal of Ethnopharmacology3.41
Deepak M. Kasote3
Estimated H-index: 3
(Aberd.: University of Aberdeen),
Suresh Jagtap7
Estimated H-index: 7
(Bharati Vidyapeeth University)
+ 2 AuthorsWendy R. Russell16
Estimated H-index: 16
(Aberd.: University of Aberdeen)
Abstract Ethanopharmacological relevance The process of formation or appearance of a urinary stone anywhere in the renal tract is known as urolithiasis. It is a longstanding health problem, known to exist since early age of civilization. Records about symptoms, signs and treatment strategies of urinary stones diseases are found in the several ancient texts of traditional medicines such as Ayurveda, Traditional Chinese Medicine (TCM), Siddha and Unani. In Ayurveda, urolithiasis has been considere...
Published on Jan 1, 2017in Pharmaceutical Biology2.49
Ikshit Sharma1
Estimated H-index: 1
(Hamdard University),
Washim Khan2
Estimated H-index: 2
(Hamdard University),
Sayeed Ahmad18
Estimated H-index: 18
(Hamdard University)
AbstractContext: Tribulus terrestris L. (Zygophyllaceae) fruits have long been used in traditional systems of medicine for the treatment of various urinary diseases including urolithiasis.Objective: To explore the anti-urolithiatic potential of gokhru and to develop an analytical method for quantitative estimation of metabolites for its quality control.Materials and methods: Aqueous extract of gokhru fruit was prepared through maceration followed by decoction to produce a mother extract, which w...
Published on Jul 1, 2016in Journal of Chromatography B2.81
Salwa Bouabdallah1
Estimated H-index: 1
(Carthage University),
Rabiaa-M. Sghaier1
Estimated H-index: 1
(Pasteur Institute)
+ 3 AuthorsMossadok Ben-Attia13
Estimated H-index: 13
(Carthage University)
In the present study, the potential effect anti tumor and the chemical composition of different fractions of Gokshur was evaluated. Commonly known as puncture vine, it has been used for a long time in both the Indian and traditional Chinese medicine. It is popularly used as a remedy for fertility disorder in Ayurveda. Samples were collected during June-September 2014 in the Cap Bon (north east of the northern Tunisia). Different organs were separated and extracted by sequential process to compar...
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