Micro diffusion cells for the growth of single protein crystals by means of equilibrium dialysis.
Published on Aug 1, 1968in Archives of Biochemistry and Biophysics3.56
· DOI :10.1016/0003-9861(68)90443-8
Abstract Microdiffusion cells are described for crystallization of small volumes of protein solutions by equilibrium dialysis against a precipitant. The cells which may contain down to 10 microliters of protein solution are made either from capillaries of glass or transparent polymer sealed with cellophane membranes or from X-ray capillaries sealed with semipermeable diaphragms of polyacrylamide. The use of these microdiffusion cells has been explored both for growing large protein crystals and for chemical treatment of one or a few single crystals in their mother liquor. The methods have been applied to lysozyme, an extracellular proteinase from a strain of Arthrobacter and to rabbit muscle aldolase. The factors influencing the growth of protein crystals are discussed and other methods for protein crystallization are briefly reviewed.