Evaluation of glucose transport and its regulation by insulin in human monocytes using flow cytometry

We investigated the effects of insulin on glucose transport in human monocytes using flow cytometry, a method with several advantages over previously used techniques. We hypothesized that monocytes could be used as tools to study insulin action at the cellular level and facilitate the investigation of mechanisms that lead to insulin resistance.Blood was withdrawn from 38 healthy subjects. The expression of glucose transporter (GLUT) isoforms in...