Substrate and Metal Complexes of 3-Deoxy-d-manno-octulosonate-8-phosphate Synthase from Aquifex aeolicus at 1.9-Å Resolution

Volume: 276, Issue: 11, Pages: 8393 - 8402
Published: Mar 1, 2001
Abstract
3-Deoxy-d-manno-octulosonate-8-phosphate synthase (KDO8PS) from the hyperthermophilic bacteriumAquifex aeolicus differs from its Escherichia coli counterpart in the requirement of a divalent metal for activity (Duewel, H. S., and Woodard, R. W. (2000)J. Biol. Chem. 275, 22824–22831). Here we report the crystal structure of the A. aeolicus enzyme, which was determined by molecular replacement using E. coli KDO8PS as a model. The structures of the...
Paper Details
Title
Substrate and Metal Complexes of 3-Deoxy-d-manno-octulosonate-8-phosphate Synthase from Aquifex aeolicus at 1.9-Å Resolution
Published Date
Mar 1, 2001
Volume
276
Issue
11
Pages
8393 - 8402
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