Combined use of AFM and X-ray diffraction to analyze crystals of an engineered, domain-deleted antibody

A genetically engineered humanized CH2-domain-deleted monoclonal antibody lacking any interchain-hinge disulfide bonds has been crystallized in the presence of detergent in a form suitable for X-ray diffraction analysis. The crystals were grown from 4 M formate along with Triton X-100 and had P21212 space-group symmetry, with unit-cell parameters a = 83, b = 224, c = 167 Å. The crystals diffract to beyond 2.8 Å resolution. A disordered crystal...