Original paper
Genetic Screens in Human Cells Using the CRISPR-Cas9 System
Abstract
Improving Whole-Genome Screens Improved methods are needed for the knockout of individual genes in genome-scale functional screens. Wang et al. (p. 80 , published online 12 December) and Shalem et al. (p. 84 , published online 12 December) used the bacterial CRISPR/Cas9 system to power-screen protocols that avoid several of the pitfalls associated with small interfering RNA (siRNA) screens. Genome editing by these methods completely disrupts...
Paper Details
Title
Genetic Screens in Human Cells Using the CRISPR-Cas9 System
Published Date
Jan 3, 2014
Journal
Volume
343
Issue
6166
Pages
80 - 84
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Notes
History