Characterisation of the Intact Rainbow Trout Vitellogenin Protein and Analysis of its Derived Tryptic and Cyanogen Bromide Peptides by Matrix-Assisted Laser Desorption/Ionisation Time-of-Flight-Mass Spectrometry and Electrospray Ionisation Quadrupole/Time-of-Flight Mass Spectrometry

Vitellogenin (VTG) is a protein produced by the liver of oviparous animals. It is being used as a biomarker for exposure to endocrine disruptors in many species. Rainbow trout Vtg has recently been sequenced by the conventional cDNA nucleotide approach. We focused on protein characterization of the intact protein and its derived tryptic and cyanogen bromide peptides by matrix-assisted laser desorption/ionisation and electrospray ionisation mass...