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Purification, structure, lipid lowering and liver protecting effects of polysaccharide from Lachnum YM281

Published on Oct 1, 2013in Carbohydrate Polymers6.04
· DOI :10.1016/j.carbpol.2013.07.014
Tao Qiu3
Estimated H-index: 3
(Hefei University of Technology),
Xiao-jing Ma2
Estimated H-index: 2
(Hefei University of Technology)
+ 2 AuthorsYanna Wu4
Estimated H-index: 4
(Hefei University of Technology)
Abstract
Abstract The extracellular polysaccharide (LEP) produced by Lachnum YM281 was obtained from the fermentation broth, and LEP-1b with molecular weight of 4.02 × 10 4  Da was separated and sequentially purified through DEAE-cellulose 52 column chromatography and Sepharose CL-6B column chromatography. GC–MS, IR and NMR ( 1 H, 13 C) spectroscopy analysis indicated that the repeat unit of LEP-1b was: Download full-size image The effects of LEP-1b on the serum lipids, liver lipids levels and aminotransferase activities of model mice with hyperlipidemic fatty live were studied, and the results showed that LEP-1b had strong lipid lowering and liver protecting effects on mice with hyperlipidemic fatty live.
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