Eight-hour PCR-based procedure for the detection ofSalmonellain raw oysters
Abstract
The aim of this study was to evaluate the ability of a nested PCR system to detect Salmonella senftenberg in raw oysters. The specific primers of the PCR were derived from the invA gene sequence, essential for Salmonella invasiveness into epithelial cells. First, for the extraction of DNA, four methods (guanidine isothiocyanate, E.Z.N.A. Mollusc Kit, Chelex-100, and lysis with detergents) were compared. A nested PCR method combined with 3.5 h...
Paper Details
Title
Eight-hour PCR-based procedure for the detection ofSalmonellain raw oysters
Published Date
Feb 1, 2005
Journal
Volume
243
Issue
1
Pages
279 - 283
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