Immobilization of thermostable nucleoside phosphorylases on MagReSyn® epoxide microspheres and their application for the synthesis of 2,6-dihalogenated purine nucleosides

Immobilization of enzymes has been considered as an efficient approach to facilitate enzyme recovery and to improve biocatalyst stability. However, multimeric nucleoside phosphorylases, useful for the synthesis of modified nucleosides, encounter several challenges to their immobilization, including requirement for high enzymatic load, and poor retention of enzyme activity. In this study, multimeric enzymes pyrimidine nucleoside phosphorylase...