Electronic ‘Off‐On’ Molecular Switch for Rapid Detection of Thrombin

An electrochemical molecular beacon aptasensor for fast and sensitive assay of thrombin is developed and characterized. A bifunctional derivative of the thrombin‐binding aptamer, 15‐base long with a redox‐active ferrocene moiety and a pendant hexanethiol linker group at the termini of the aptamer strand was immobilized on Au electrode surface. The electrochemical behavior of the aptasensor was studied using cyclic voltammetry (CV),...