Single-step purification of recombinant green fluorescent protein on expanded beds of immobilized metal affinity chromatography media
Abstract
Immobilized metal ion affinity chromatography (IMAC) in expanded bed mode is used for purifying recombinant green fluorescent protein (GFP) overexpressed in Escherichia coli. The purification is carried out on two different matrices, i.e. Ni2+ Streamline™ and Ni2+ cross-linked alginate beads. The binding isotherms to both IMAC media followed the Langmuir model. The maximum binding capacity (qmax) of Ni2+ Streamline™ and Ni2+ cross-linked...
Paper Details
Title
Single-step purification of recombinant green fluorescent protein on expanded beds of immobilized metal affinity chromatography media
Published Date
Dec 1, 2008
Volume
42
Issue
3
Pages
301 - 307
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