An extracellular proteolytic enzyme from a strain of Arthrobacter II. Purification and chemical properties of the enzyme

A new extracellular proteolytic enzyme has been isolated from the culture medium of a strain of Arthrobacter grown in the presence of gelatin. The fractionation procedure makes use of precipitation with ammonium sulfate in the presence of cellulose, treatment with DEAE-Sephadex, gel filtration on Sephadex G-100 and crystallisation with ammonium sulfate or acetone. Ultracentrifugation of the crystallized enzyme showed only one sedimenting...