Chemical characterization and antifungal activity of Cinnamomum camphora essential oil
Abstract Chiral compounds have shown antimicrobial activity, enantioselectively. Leaf oil composition of Cinnamomum camphora was studied using GC-FID, SPME-GC-FID, enantio-GC and GC/MS techniques. Camphor exists in two chiral forms and contributes major percentage to C. camphora essential oil (68.4–81.2%). The results showed (1 R )-(+)-camphor as major enantiomer ( ee > 99%). The biological potential of essential oil, (1 R )-(+)- and (1 S )-(−)-camphor was investigated against Choanephora cucurbitarum , a Withania wet rot pathogen. The activity was evaluated against fungal growth and morphological changes in the hyphae of C. cucurbitarum , after 18, 21 and 24 h of treatments. A significant growth inhibition in C. cucurbitarum was observed against (1 R )-(+)-camphor. Further, in comparison to (1 R )-(+)-camphor, C. camphora oil revealed better activity. Fungal growth inhibition by (1 R )-(+)-camphor and plant essential oil was due to cytoplasm coagulation and hyphal lysis of C. cucurbitarum . Moreover, C. camphora essential oil could be used in the preparation of strong fungistatic agent against C. cucurbitarum infection.