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Double-helical RNA in satellite tobacco mosaic virus

Published on Jan 1, 1993in Nature43.07
· DOI :10.1038/361179a0
Steven B. Larson13
Estimated H-index: 13
(UCR: University of California, Riverside),
Stanley Koszelak12
Estimated H-index: 12
(UCR: University of California, Riverside)
+ 3 AuthorsAlexander McPherson53
Estimated H-index: 53
(UCR: University of California, Riverside)
Abstract
SATELLITE tobacco mosaic virus (STMV) is the spherical satellite to an obligatory rod-shaped helper tobacco mosaic virus (TMV), which is required for replication1,2. STMV has 60 protein subunits of Mr 17,500 on a T = 1 icosahedral capsid3 containing a single stranded RNA genome of 1,059 bases4–6. STMV appears similar to another virus, STNV7,8, but is ~20 per cent smaller. It shows no amino-acid homology or immunological cross-reactivity with either STNV or its host TMV4,9. Here we report the X-ray crystal structure of STMV, which shows that the coat protein of STMV contains a ‘Swiss roll’ β-barrel. An amino-terminal strand extends more than 60A and is primarily responsible for quaternary interactions. Each capsid dimer is associated with a segment of genomic RNA double helix comprising seven base pairs. The dyad of each protein dimer is coincident with that of the central base pair of the associated RNA segment whose helix axis is directed along an icosahedral edge. Protein-nucleic acid interactions are extensive. The RNA helices, which have additional stacked bases at their 3′ termini, differ significantly from canonical nucleic acid helical forms.
  • References (35)
  • Citations (83)
References35
Newest
#1John Day (UCR: University of California, Riverside)H-Index: 15
#2Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
#1Robert McKenna (Purdue University)H-Index: 8
#2Di Xia (Warw.: University of Warwick)H-Index: 4
Last.Nino L. Incardona (UTHSC: University of Tennessee Health Science Center)H-Index: 10
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#1Jun Tsao (Purdue University)H-Index: 14
#2Michael S. Chapman (Purdue University)H-Index: 35
Last.Colin R. Parrish (Cornell University)H-Index: 53
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#1K.S. Kim (UA: University of Arkansas)H-Index: 12
#2Rodrigo A. Valverde (LSU: Louisiana State University)H-Index: 20
Last.J. Allan Dodds (UCR: University of California, Riverside)H-Index: 10
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#1Stanley Koszelak (UCR: University of California, Riverside)H-Index: 12
#2James Allan Dodds (UCR: University of California, Riverside)H-Index: 1
Last.Alexander McPherson (UCR: University of California, Riverside)H-Index: 53
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#1Adam ZlotnickH-Index: 46
#2Samson FrancisH-Index: 6
Last.Joseph Che-Yen WangH-Index: 15
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#1Rees F. Garmann (UCLA: University of California, Los Angeles)H-Index: 11
#2Ajaykumar Gopal (UCLA: University of California, Los Angeles)H-Index: 13
Last.Stephen C. Harvey (Georgia Institute of Technology)H-Index: 45
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#1James A. Geraets (Ebor: University of York)H-Index: 2
#2Eric C. Dykeman (Ebor: University of York)H-Index: 17
Last.Reidun Twarock (Ebor: University of York)H-Index: 22
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View next paperRefined structure of satellite tobacco mosaic virus at 1.8 A resolution.