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Clustering phenomenon and growth units in lysozyme aqueous solution as revealed by laser light scattering method

Published on Nov 1, 1989in Journal of Crystal Growth1.573
· DOI :10.1016/0022-0248(89)90153-X
T. Azuma1
Estimated H-index: 1
(Tohoku University),
K. Tsukamoto16
Estimated H-index: 16
(Tohoku University),
Ichiro Sunagawa27
Estimated H-index: 27
(Tohoku University)
Abstract
Abstract To analyse the clustering phenomenon, the diffusion coefficient in and the viscosity of the bulk supersaturated solutions were measured using quasi-elastic light scattering, and the concentration gradient in the diffusion boundary layer around a growing crystal interface was measured by means of Mach-Zehnder interferometry. The results obtained in case of tetragonal lysozyme crystals have demonstrated that (1) the solute particle size determined from values of both the diffusion coefficient and the viscocity indicated the presence of the solute clustering in the bulk supersaturated solutions, but (2) the major growth units controlling the growth rate were monomolecular entities. The growth kinetics for tetragonal lysozyme crystals were also analysed, based on the surface microtopographic observations, and it was shown that the growth was principally controlled by the surface kinetic process.
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Abstract A series of preliminary experiments were done to investigate solutal flow about growing lysozyme crystals and its effects. Density gradient driven flow as observed using a schlieren optical system. Crystals used ranged from 0.3 to 1.72 mm across the (110) face, and protein concentrations were from 3.7 to 23.7 mg/ml ( C sat = 1.2 mg/ml at 18°C). The convective plume velocities were found to be from 10 to 50 μm/s, which correlated with those predicted to occur based upon a diffusive-conve...
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Abstract Using Schlieren technique and Mach-Zehnder interferometry, the diffusion boundary layer and buoyancy driven convection around a growing barium nitrate crystal from the aqueous solution were visualized and their effect upon the growth kinetics of crystals was investigated in relation to the bulk supersaturation. It was demonstrated that buoyancy driven convection plumes behaved differently depending on the bulk supersaturation, and that their behavior gave a definitive effect upon the gr...
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