Development and validation of a real-time Taqman® PCR assay for the detection and quantitation of infectious laryngotracheitis virus in poultry

In this study, the development and validation of a real-time (ReTi) PCR assay is described using a Taqman® labeled probe for the detection and quantitation of infectious larygotracheitis virus (ILTV) in chickens. The ReTi ILTV assay was highly specific with a quantitation limit of 100 viral template copies per amplification reaction. In experimentally infected, birds during early acute stages of infection, an average of 6.67 log10 viral template...