A direct method for fatty acid methyl ester synthesis: application to wet meat tissues, oils, and feedstuffs.

Volume: 85, Issue: 6, Pages: 1511 - 1521
Published: Jun 1, 2007
Abstract
A simplified protocol to obtain fatty acid methyl esters (FAME) directly from fresh tissue, oils, or feedstuffs, without prior organic solvent extraction, is presented. With this protocol, FAME synthesis is conducted in the presence of up to 33% water. Wet tissues, or other samples, are permeabilized and hydrolyzed for 1.5 h at 55 degrees C in 1 N KOH in MeOH containing C13:0 as the internal standard. The KOH is neutralized, and the FFA are...
Paper Details
Title
A direct method for fatty acid methyl ester synthesis: application to wet meat tissues, oils, and feedstuffs.
Published Date
Jun 1, 2007
Volume
85
Issue
6
Pages
1511 - 1521
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