Comparison of cytochrome P450 concentrations and metabolic activities in porcine hepatic microsomes prepared with two different methods

Volume: 25, Issue: 1, Pages: 343 - 346
Published: Feb 1, 2011
Abstract
In the present study, porcine liver microsomes prepared by a conventional ultracentrifugation method were compared with microsomes prepared by a calcium aggregation method. Protein concentrations and activities of several cytochrome P450 enzymes were measured. It was concluded that using a calcium aggregation method for microsome preparation resulted in lower activities of porcine 7-ethoxyresorufin O-deethylase (EROD), 7-methoxyresorufin...
Paper Details
Title
Comparison of cytochrome P450 concentrations and metabolic activities in porcine hepatic microsomes prepared with two different methods
Published Date
Feb 1, 2011
Volume
25
Issue
1
Pages
343 - 346
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