Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
Abstract
The simplicity of programming the CRISPR (clustered regularly interspaced short palindromic repeats)–associated nuclease Cas9 to modify specific genomic loci suggests a new way to interrogate gene function on a genome-wide scale. We show that lentiviral delivery of a genome-scale CRISPR-Cas9 knockout (GeCKO) library targeting 18,080 genes with 64,751 unique guide sequences enables both negative and positive selection screening in human cells....
Paper Details
Title
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
Published Date
Jan 3, 2014
Journal
Volume
343
Issue
6166
Pages
84 - 87
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