Amperometric Biosensor for Evaluation of Competitive Cholinesterase Inhibition by the Reactivator HI‐6

Amperometric biosensors containing enzymes butyrylcholinesterase or acetylcholinesterase were prepared. The biosensors were employed for studying of cholinesterase reactivator: HI‐6. Competitions between HI‐6 and acetylthiocholine as enzyme substrate were used for determination of IC50 value. Biosensors with butyrylcholinesterase from human serum determined IC50 as (1.00±0.02)×10−6 M; the biosensor with acetylcholinesterase from human...