On Mechanism of Intermediate-Sized Circular DNA Compaction Mediated by Spermine: Contribution of Fluorescence Lifetime Correlation Spectroscopy

The compaction of DNA plays a role in the nuclei of several types of cells and becomes important in the non-viral gene therapy. Thus, it is in the scope of research interest. It was shown, that spermine-induced compaction of large DNA molecules occurs in a discrete all-or-non regime, where the coexistence of free and folded DNA molecules was observed. In the case of intermediate-sized DNA molecules (approximately 10 kbp), so far, it was stated...