Chemical and Genetic Studies on Hybrid ofLigularia subspicataandLigularia cyathicepsCollected in Yunnan Province of China

Two morphologically ambiguous Ligularia samples (samples A and B ), and samples with morphology of Ligularia subspicata (sample C ), Ligularia lamarum (sample D ), or Ligularia cyathiceps (sample E ), were collected at Tianchi Pond, Shangrila County, Yunnan Province, China. Analysis of the nucleotide sequence of the internal transcribed spacers (ITSs) in the nuclear ribosomal RNA gene cluster indicated that not only sample B but also sample D...