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Antioxidant Activities of the Chloroform Extract of Solanum trilobatum

Published on Jan 1, 2004in Pharmaceutical Biology2.49
· DOI :10.1080/13880200490886238
H. Sini1
Estimated H-index: 1
(UoK: University of Kerala),
K.S. Devi1
Estimated H-index: 1
(UoK: University of Kerala)
Cite
Abstract
A chloroform extract from the anticancer herb Solanum trilobatum (CST) (Nadkarni, 1979) was analyzed and compared with reference antioxidants for its in vitro antioxidative properties such as scavenging of α,α-diphenyl-β-picryl hydrazyl (DPPH) and superoxide radicals, protection to deoxyribose degradation, reducing power, as well as inhibition of lipid peroxidation. Preliminary phytochemical analysis of CST by silica gel thin-layer chromatography showed the presence of simple phenols, phenolic acids, isoflavones, xanthones, and lignans. The antioxidative effect of CST was found to be concentration dependent to a certain extent and then leveled off with further increase in concentration. The IC50 for each antioxidative reaction studied was calculated. When compared to the reference antioxidant butylated hydroxytoluene (BHT), CST exhibited less scavenging effect on DPPH radicals and reducing power but a better superoxide radical scavenging effect. From a comparison of the hydroxyl radical scavenging effect ...
  • References (20)
  • Citations (25)
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References20
Newest
Published on Jan 1, 2002in Journal of Agricultural and Food Chemistry3.57
Santosh Khokhar13
Estimated H-index: 13
(University of Leeds),
Steinunn Magnusdottir1
Estimated H-index: 1
(University of Leeds)
Levels of total phenol, catechins, and caffeine in teas commonly consumed in the United Kingdom have been determined using reversed phase high-performance liquid chromatography. Tea bags or tea leaves were purchased from local supermarkets and extracted in boiling water for 5 min. The resulting data showed considerable variability in both total phenols [80.5−134.9 mg/g of dry matter (DM) in black teas and 87−106.2 mg/g of DM in green teas] and catechins (5.6−47.5, 51.5−84.3, and 8.5−13.9 mg/g of...
Published on Nov 30, 2000in Toxicology3.55
J.P. Kamat15
Estimated H-index: 15
(BARC: Bhabha Atomic Research Centre),
T.P.A. Devasagayam37
Estimated H-index: 37
(BARC: Bhabha Atomic Research Centre)
Cellular damage induced by reactive oxygen species (ROS) in normal tissues has been implicated in the etiology of several human ailments. Among the subcellular organelles, damage to mitochondria is considered crucial and can lead to cytotoxicity and cell death. However, the same damage, if it is selectively induced in cancer tissues can lead to its cure. Hence analyzing the mechanisms of such damage and its modulation may result in better prevention or cure. Using mitochondria derived from rat b...
Published on Nov 1, 2000in Nature43.07
Toren Finkel85
Estimated H-index: 85
(LMB: Laboratory of Molecular Biology),
Nikki J. Holbrook64
Estimated H-index: 64
(NIH: National Institutes of Health)
Living in an oxygenated environment has required the evolution of effective cellular strategies to detect and detoxify metabolites of molecular oxygen known as reactive oxygen species. Here we review evidence that the appropriate and inappropriate production of oxidants, together with the ability of organisms to respond to oxidative stress, is intricately connected to ageing and life span.
Published on Aug 1, 2000in Journal of Agricultural and Food Chemistry3.57
Raquel Pulido5
Estimated H-index: 5
,
Laura Bravo44
Estimated H-index: 44
,
Fulgencio Saura-Calixto59
Estimated H-index: 59
Most nonenzymatic antioxidant activity (scavenging of free radicals, inhibition of lipid peroxidation, etc.) is mediated by redox reactions. The antioxidant (AO) activity of polyphenols (PPs), as ferric-reducing power, was determined for the first time using a modified FRAP (ferric reducing/antioxidant power) assay. Reaction was followed for 30 min, and both Fe(II) standards and samples were dissolved in the same solvent to allow comparison. Selected representative PPs included flavonoids (querc...
Published on Jan 1, 2000in Pharmacological Reviews18.89
Elliott Middleton32
Estimated H-index: 32
,
Chithan Kandaswami17
Estimated H-index: 17
,
Theoharis C. Theoharides73
Estimated H-index: 73
Flavonoids are nearly ubiquitous in plants and are recognized as the pigments responsible for the colors of leaves, especially in autumn. They are rich in seeds, citrus fruits, olive oil, tea, and red wine. They are low molecular weight compounds composed of a three-ring structure with various substitutions. This basic structure is shared by tocopherols (vitamin E). Flavonoids can be subdivided according to the presence of an oxy group at position 4, a double bond between carbon atoms 2 and 3, o...
Published on Jan 1, 1999in Methods in Enzymology1.86
Vernon L. Singleton1
Estimated H-index: 1
,
Rudolf Orthofer1
Estimated H-index: 1
,
Rosa M. Lamuela-Raventós71
Estimated H-index: 71
Publisher Summary This chapter discusses the analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Analyses of the Folin-Ciocalteu (FC) type are convenient, simple, and require only common equipment and have produced a large body of comparable data. Under proper conditions, the assay is inclusive of monophenols and gives predictable reactions with the types of phenols found in nature. Because different phenols react to different degrees, e...
Published on Jan 1, 1998in Bioscience, Biotechnology, and Biochemistry1.30
Tomoko Yamaguchi12
Estimated H-index: 12
(Nara Women's University),
Hitoshi Takamura16
Estimated H-index: 16
(Nara Women's University)
+ 1 AuthorsJunji Terao56
Estimated H-index: 56
(University of Tokushima)
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. ...
Chung-Shi Yang13
Estimated H-index: 13
(PC: Providence College),
Pi-Ju Tsai11
Estimated H-index: 11
+ 3 AuthorsJon-Son Kuo26
Estimated H-index: 26
Abstract A method for in vivo evaluation of lipid peroxidation in the extracellular space of anaesthetized rat brain cortex was developed. This method involved the use of microdialysis perfusion and high-performance liquid chromatography. The microdialysates, eluted from implanted probes, were reacted with thiobarbituric acid (TBA) prior to analysis by an HPLC system equipped with a fluorescence detector (excitation and emission wavelengths were 515 and 550 nm, respectively). Lipid peroxidation ...
Published on Jan 1, 1997in Cancer Letters6.51
P. Mohanan28
Estimated H-index: 28
,
K.S. Devi6
Estimated H-index: 6
(UoK: University of Kerala)
The partially purified component of Solanum trilobatum named Sobatum was obtained from the petroleum ether/ethyl acetate (75:25) extractable portion. It was found to be cytotoxic in Dalton's Lymphoma ascites (DLA), Ehrlich ascites (EA) cell lines and tissue culture cells (L929 and Vero). Sobatum significantly inhibited peritoneal tumours induced by DLA and EA tumour cells. Sobatum was also found to reduce solid tumour growth in mice, when given either simultaneously or prophylactically, and is m...
Published on Dec 1, 1996in Cancer Letters6.51
P. Mohanan28
Estimated H-index: 28
,
K.S. Devi6
Estimated H-index: 6
(UoK: University of Kerala)
Abstract Plant Solanum trilobatum was washed, powdered and used for extraction. The lyophilized aqueous extracted portion was tested for in vitro cytotoxicity by tissue culture technique using L929 and Vero cells. Petroleum ether, chloroform, ethyl acetate and ethanol were used for extraction and the extracted portions were subjected to in vitro tissue culture studies. It was shown that petroleum ether extract induced remarkable cytotoxicity, when compared to all other extracts with an LD 50 of ...
Cited By25
Newest
Published on Dec 1, 2018in Scientific Reports4.01
Adil Lateef , Sudheesh K. Prabhudas2
Estimated H-index: 2
,
Purushothaman Natarajan3
Estimated H-index: 3
Solanum trilobatum L. is an important medicinal plant in traditional Indian system of medicine belonging to Solanaceae family. However, non-availability of genomic resources hinders its research at the molecular level. We have analyzed the S. trilobatum leaf transcriptome using high throughput RNA sequencing. The de novo assembly of 136,220,612 reads produced 128,934 non-redundant unigenes with N50 value of 1347 bp. Annotation of unigenes was performed against databases such as NCBI nr database,...
Published on Sep 1, 2018
T. Muralisankar (BDU: Bharathidasan University), P. Saravana Bhavan8
Estimated H-index: 8
(Bharathiar University)
+ 1 AuthorsP. Santhanam7
Estimated H-index: 7
(BDU: Bharathidasan University)
Experiments were conducted to evaluate the influence of dietary supplement (@ 5%) dried leaf powder of the medicinal herbs, Ocimum sanctum, Solanum trilobatum and Phyllanthus amarus on survival, growth, activities of digestive enzymes, energy utilization, and vitamin contents of the freshwater prawn, Macrobrachium rosenbergii. Results indicated significant (P < 0.05) improvements in survival, growth, activities of digestive enzymes, energy utilization, and vitamin levels in herb supplemented fee...
Published on Aug 22, 2017in Antioxidants
Kumar Ganesan4
Estimated H-index: 4
,
Kumeshini Sukalingam3
Estimated H-index: 3
,
Baojun Xu29
Estimated H-index: 29
Solanum trilobatum L. (Solanaceae) has been well known as nightshade, commonly used by diverse populations to heal several disorders. Earlier studies in Solanum trilobatum were focused on different pharmacological activities and a few were concerned with antioxidant and hepatoprotective effects. Thus, the current study was focused to evaluate the antioxidant potential and hepatoprotective effects of S. trilobatum L. on thioacetamide (TAA) intoxication in Wistar albino rats. The rats were kept in...
H. Sini1
Estimated H-index: 1
(UoK: University of Kerala),
K.S. Devi1
Estimated H-index: 1
(UoK: University of Kerala),
K.G. Nevin1
Estimated H-index: 1
(UoK: University of Kerala)
The aim of this study was to assess the potential of chloroform extract of S. trilobatum (CST) on 7,12-dimethyl benz (a)anthracene (DMBA) induced skin carcinogenesis and Ehrlich ascites carcinoma cells (EAC) induced solid tumor in mice. CST was obtained by exhaustive extraction with chloroform. Skin carcinogenesis was induced in Swiss albino mice by applying DMBA, while solid tumor was induced by intramuscular injection of EAC cells. For preventing skin carcinogenesis, animals were treated with ...
Published on Feb 1, 2017in Cytotechnology1.67
Shridhar C Ghagane1
Estimated H-index: 1
(Karnatak University),
Shridevi I. Puranik (Karnatak University)+ 1 AuthorsMurigendra B. Hiremath8
Estimated H-index: 8
(Karnatak University)
Allophylus cobbe (L.) Raeusch. belonging to the family Sapindaceae, is a commonly distributed small shrub in Western Ghats of India previously reported for its traditional medicinal properties. It is used for the treatment of various ailments. The present study is aimed at investigating preliminary phytochemicals, inducing the determination of the total phenolic contents, antioxidant assays and anticancer activity of A. cobbe leaf extracts on (DU-145) and (PC-3) cell lines. Preliminary phytochem...
Published on Dec 1, 2016in BMC Complementary and Alternative Medicine2.48
Umbreen Rashid7
Estimated H-index: 7
,
Muhammad Rashid Khan18
Estimated H-index: 18
(QAU: Quaid-i-Azam University),
Moniba Sajid7
Estimated H-index: 7
(QAU: Quaid-i-Azam University)
Background Fagonia olivieri (DC) being used for the treatment of diabetes, cancer, fever and claimed to be effective in many other stress related disorders. In this study we have evaluated the F. olivieri whole methanol extract and its derived fractions for various in vitro and in vivo antioxidant studies.
Published on Aug 1, 2016in Cytotechnology1.67
Marijana Kosanić14
Estimated H-index: 14
(University of Kragujevac),
Branislav Ranković18
Estimated H-index: 18
(University of Kragujevac)
+ 3 AuthorsOlivera Milosevic-Djordjevic8
Estimated H-index: 8
(University of Kragujevac)
The methanol extract of the lichen Lasallia pustulata was tested for genotoxic, antioxidant, antimicrobial and anticancer activities. We did this using a cytokinesis block micronucleus (MN) assay on peripheral blood lymphocytes, by measuring free radical and superoxide anion scavenging activity, reducing power, determining of total phenolic compounds and determining the total flavonoid content, measuring the minimal inhibitory concentration by the broth microdilution method against five species ...
Published on Sep 1, 2015in Phytochemistry Letters1.34
Lan Li6
Estimated H-index: 6
,
Qin-Peng Shen6
Estimated H-index: 6
+ 9 AuthorsZhi-Hua Liu7
Estimated H-index: 7
Abstract Three new isoflavones, 4′,8-dihydroxy-6,7-dimethoxyisoflavone ( 1 ), 4′,6-dihydroxy-8-methoxycarbonyl-7-methoxyisoflavone ( 2 ), 4′,7-dimethoxy-8-hydroxymethyl-6-hydroxyisoflavone ( 3 ), together with three known flavones ( 4 – 6 ), were isolated from the leaves of Nicotiana tabacum . Their structures were elucidated by spectroscopic methods, including extensive 1D- and 2D NMR techniques. Compounds 1 – 3 were evaluated for their anti-tobacco mosaic virus (anti-TMV) activities. The resul...