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Isolation and chemical characterization of a glucogalactomannan of the medicinal mushroom Cordyceps militaris

Published on Aug 1, 2013in Carbohydrate Polymers6.044
· DOI :10.1016/j.carbpol.2013.04.049
Fhernanda R. Smiderle18
Estimated H-index: 18
(UFPR: Federal University of Paraná),
Guilherme L. Sassaki37
Estimated H-index: 37
(UFPR: Federal University of Paraná)
+ 1 AuthorsMarcello Iacomini39
Estimated H-index: 39
(UFPR: Federal University of Paraná)
Abstract
Cordyceps militaris dried fruiting bodies were extracted with 5% KOH solution. The extract was purified by freeze-thawing treatment, and dialysis (100 kDa), giving rise to a homogeneous polysaccharide (M-w 23,000 Da). Its monosaccharide composition was mannose (56.7%), galactose (34.5%), and glucose (8.8%). The anomeric configurations were determined by their coupling constants. A complex polysaccharide was identified by NMR and methylation analysis. The HSQC spectrum showed signals at delta 107.7/5.06 and 106.1/5.14; 105.9/5.12 relative to beta-D-Galf, and O-2-substituted beta-D-Galf units, respectively. The sign at delta 104.4/5.21 corresponded to alpha-D-Galf. Other signals corresponded to alpha-D-Manp O-6- and O-2-substituted (delta 100.2/4.94; 100.5/5.27; 100.6/5.23; 100.7/5.16), and alpha-D-Manp 2,6-di-O-substituted (from delta 99.3 to 99.9). The main linkages, confirmed by methylation analysis, showed the derivatives: 2,3,4-Me-3-Manp (11.9%) and 3,4,6-Me-3-Manp (28.6%). The branches were (1 -> 6)-linked-alpha-D-Manp or (1 -> 2)-linked-beta-D-Galf, terminating with beta-D-Galf, alpha-D-Galf, alpha-D-Galp, or alpha-D-Manp. 42.7% of the partially hydrolyzed product consisted of 3,4,6-Me-3-Manp, suggesting a (1 -> 2)-linked backbone. (C) 2013 Elsevier Ltd. All rights reserved.
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