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Isolation and chemical characterization of a glucogalactomannan of the medicinal mushroom Cordyceps militaris

Published on Aug 1, 2013in Carbohydrate Polymers6.04
· DOI :10.1016/j.carbpol.2013.04.049
Fhernanda R. Smiderle17
Estimated H-index: 17
(UFPR: Federal University of Paraná),
Guilherme L. Sassaki34
Estimated H-index: 34
(UFPR: Federal University of Paraná)
+ 1 AuthorsMarcello Iacomini38
Estimated H-index: 38
(UFPR: Federal University of Paraná)
Abstract
Abstract Cordyceps militaris dried fruiting bodies were extracted with 5% KOH solution. The extract was purified by freeze-thawing treatment, and dialysis (100 kDa), giving rise to a homogeneous polysaccharide ( M w 23,000 Da). Its monosaccharide composition was mannose (56.7%), galactose (34.5%), and glucose (8.8%). The anomeric configurations were determined by their coupling constants. A complex polysaccharide was identified by NMR and methylation analysis. The HSQC spectrum showed signals at δ 107.7/5.06 and 106.1/5.14; 105.9/5.12 relative to β- d -Gal f , and O-2-substituted β- d -Gal f units, respectively. The sign at δ 104.4/5.21 corresponded to α- d -Gal f . Other signals corresponded to α- d -Man p O-6- and O-2-substituted ( δ 100.2/4.94; 100.5/5.27; 100.6/5.23; 100.7/5.16), and α- d -Man p 2,6-di- O -substituted (from δ 99.3 to 99.9). The main linkages, confirmed by methylation analysis, showed the derivatives: 2,3,4-Me 3 -Man p (11.9%) and 3,4,6-Me 3 -Man p (28.6%). The branches were (1 → 6)-linked-α- d -Man p or (1 → 2)-linked-β- d -Gal f , terminating with β- d -Gal f , α- d -Gal f , α- d -Gal p , or α- d -Man p . 42.7% of the partially hydrolyzed product consisted of 3,4,6-Me 3 -Man p , suggesting a (1 → 2)-linked backbone.
  • References (52)
  • Citations (27)
References52
Newest
#1Fhernanda R. Smiderle (UFPR: Federal University of Paraná)H-Index: 17
#2Giovana Alquini (UFPR: Federal University of Paraná)H-Index: 4
Last.Leo J.L.D. Van Griensven (WUR: Wageningen University and Research Centre)H-Index: 25
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#1Cristiane Hatsuko Baggio (UFPR: Federal University of Paraná)H-Index: 27
#2Cristina Setim Freitas (UFPR: Federal University of Paraná)H-Index: 19
Last.Adair R.S. Santos (UFSC: Universidade Federal de Santa Catarina)H-Index: 55
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#1Jaung Geng Lin (PRC: China Medical University (PRC))H-Index: 29
#2Ming Jen Fan (AU: Asia University (Taiwan))H-Index: 15
Last.W. Gibson Wood (UMN: University of Minnesota)H-Index: 43
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#1Wasaporn ChanputH-Index: 8
#2Marit Reitsma (WUR: Wageningen University and Research Centre)H-Index: 6
Last.Harry J. Wichers (WUR: Wageningen University and Research Centre)H-Index: 37
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#1Lu Ren (University of Auckland)H-Index: 4
#2Conrad O. Perera (University of Auckland)H-Index: 31
Last.Yacine Hemar (University of Auckland)H-Index: 30
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#1Rolf Jorde (UNN: University Hospital of North Norway)H-Index: 54
#2Monica Sneve (UNN: University Hospital of North Norway)H-Index: 14
Last.Guri Grimnes (UNN: University Hospital of North Norway)H-Index: 29
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Cited By27
Newest
#1Claudia Rejane Lima de Macedo Costa (UNIOESTE: State University of West Paraná)H-Index: 4
#2Rafael Andrade Menolli (UNIOESTE: State University of West Paraná)H-Index: 2
Last.José Luis da Conceição Silva (UNIOESTE: State University of West Paraná)H-Index: 5
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#1Adnan Saeed (PAN: Polish Academy of Sciences)
#2Mariola Paściak (PAN: Polish Academy of Sciences)H-Index: 9
Last.Andrzej Gamian (PAN: Polish Academy of Sciences)H-Index: 26
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#1Shayane da Silva Milhorini (UFPR: Federal University of Paraná)H-Index: 1
#2Fhernanda R. Smiderle (UFPR: Federal University of Paraná)H-Index: 17
Last.Marcello Iacomini (UFPR: Federal University of Paraná)H-Index: 38
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#1Ping Xu (Hefei University of Technology)H-Index: 4
#2Ruyue Yuan (Hefei University of Technology)H-Index: 5
Last.Ming Ye (Hefei University of Technology)H-Index: 14
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