Match!

Epidemiological survey and molecular characterization of avian infectious bronchitis virus in Brazil between 2003 and 2009

Published on Apr 1, 2011in Avian Pathology1.96
· DOI :10.1080/03079457.2010.544641
Jorge Luis Chacón10
Estimated H-index: 10
(USP: University of São Paulo),
Juliana Nogueira Rodrigues2
Estimated H-index: 2
(USP: University of São Paulo)
+ 3 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
Cite
Abstract
As part of an epidemiological study of infectious bronchitis virus (IBV) in Brazil, 252 samples from IBV-suspect flocks were tested and the IBV-positive samples were analysed by sequencing of hypervariable regions 1 and 2 of the S1 gene. A high prevalence of IBV variants was found and the sequence analysis of 41 samples revealed a high molecular similarity among the Brazilian isolates (from 90.2 to 100% and from 85.3 to 100% nucleotide and amino acid identity, respectively). The Brazilian isolates showed low genetic relationship with Massachusetts (63.4 to 70.7%), European (45.9 to 75.6%), American (49.3 to 76.4%) and other reference serotypes (67.5 to 78.8%). The Brazilian isolates branched into one unique cluster, separate from the reference serotypes used for infectious bronchitis control in other countries. The variants analysed in this work had a high similarity with all previously published Brazilian IBV isolates, suggesting the presence and high prevalence of a unique or predominant genotype circul...
  • References (30)
  • Citations (27)
Cite
References30
Newest
Published on Apr 1, 2009in Avian Pathology1.96
Agustina Rimondi9
Estimated H-index: 9
(INTA: International Trademark Association),
María Isabel Craig13
Estimated H-index: 13
(INTA: International Trademark Association)
+ 3 AuthorsAriel Pereda17
Estimated H-index: 17
(INTA: International Trademark Association)
Twenty infectious bronchitis virus isolates were recovered from broilers and layers in different outbreaks amongst commercial poultry flocks in different geographic regions of Argentina from 2001 to 2008. The viruses were isolated from the tracheas, lungs, and caecal tonsils of birds that were showing respiratory signs. Further analysis based on their nucleotide and amino acid sequences in hypervariable region (HVR) 1 and the intervening sequence including HVRs 1 and 2 (HVR1/2) of the S1 gene wa...
Published on Jun 1, 2008in Avian Pathology1.96
K. J. Worthington10
Estimated H-index: 10
(University of Liverpool),
R. J. W. Currie1
Estimated H-index: 1
,
R. C. Jones35
Estimated H-index: 35
(University of Liverpool)
A survey of infectious bronchitis virus (IBV) genotypes in poultry flocks in selected countries in Western Europe was carried out between March 2002 and December 2006. Identification of IBV was by reverse transcriptase-polymerase chain reaction of RNA extracted from oropharyngeal swabs taken from poultry flocks exhibiting signs of clinical disease thought to be attributable to IBV. Part of the hypervariable S1 gene of IBV was sequenced to differentiate between the various genotypes. During the s...
Published on Dec 1, 2007in Avian Diseases1.31
L. Y. B. Villarreal7
Estimated H-index: 7
,
Paulo Eduardo Brandão18
Estimated H-index: 18
(USP: University of São Paulo)
+ 6 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
Abstract The pathogenesis of infection involving both infectious bronchitis virus (IBV) and avian metapneumovirus (aMPV) causes reproductive damage in hens after viral replication in the epithelium of the oviduct, resulting in loss of cilia and degeneration and necrosis of the epithelial and glandular cells. Although IBV has been indicated as a possible cause of the formation of calcium stones in the epididymus of roosters, a definitive association has not been confirmed. This report describes t...
Published on Dec 1, 2007in Avian Diseases1.31
L. Y. B. Villarreal7
Estimated H-index: 7
,
Paulo Eduardo Brandão18
Estimated H-index: 18
(USP: University of São Paulo)
+ 4 AuthorsA. J. P. Ferreira13
Estimated H-index: 13
Abstract Infectious bronchitis virus (IBV) is the causative agent of avian infectious bronchitis, which is characterized by respiratory, reproductive, and renal signs. However, the role of IBV as an enteric pathogen in still controversial. In Brazil, antigenic groups of IBV divergent from the Massachusetts serotype used for vaccination schedules in that country have already been demonstrated. The present study aimed to assess the different genotypes of IBV in Brazilian commercial poultry flocks ...
Published on Oct 1, 2006in Avian Pathology1.96
Yury A. Bochkov20
Estimated H-index: 20
,
Galina V. Batchenko2
Estimated H-index: 2
+ 2 AuthorsVladimir V. Drygin6
Estimated H-index: 6
Molecular characterization of infectious bronchitis viruses (IBVs) isolated between 1998 and 2002 from chickens in Russia was performed. More than 250 field samples were tested by reverse transcriptase-polymerase chain reaction using two sets of primers corresponding to the most conserved 3′-untranslated region and the most variable S1 gene region of the viral genome. Ninety-one IBV isolates were characterized by phylogenetic analysis of the S1 gene hypervariable region comprising 136 to 558 nuc...
Published on Aug 1, 2006in Archives of Virology2.26
J. Ignjatovic19
Estimated H-index: 19
(AAHL: Australian Animal Health Laboratory),
G. Gould6
Estimated H-index: 6
(AAHL: Australian Animal Health Laboratory),
S. Sapats12
Estimated H-index: 12
(AAHL: Australian Animal Health Laboratory)
Australian infectious bronchitis viruses (IBV) have undergone a separate evolution due to geographic isolation. Consequently, changes occurring in Australian IBV illustrate, independently from other countries, types of variability that could occur in emerging IBV strains. Previously, we have identified two distinct genetic groups of IBV, designated subgroups 1 and 2. IBV strains of subgroup 1 have S1 and N proteins that share a high degree of amino acid identity, 81 to 98% in S1 and 91 to 99% in...
Published on Apr 1, 2006in Avian Pathology1.96
Roser Dolz12
Estimated H-index: 12
,
Joan Pujols25
Estimated H-index: 25
+ 2 AuthorsNatàlia Majó20
Estimated H-index: 20
(Autonomous University of Barcelona)
As part of an epidemiological surveillance of infectious bronchitis virus (IBV) in Spain, four Spanish field isolates showed high S1 spike sequence similarities with an IBV sequence from the GenBank database named Italy 02. Given that little was known about this new emergent IBV strain we have characterized the four isolates by sequencing the entire S1 part of the spike protein gene and have compared them with many reference IBV serotypes. In addition, cross-virus neutralization assays were cond...
Published on Apr 1, 2006in Avian Pathology1.96
B. S. Ladman14
Estimated H-index: 14
(UD: University of Delaware),
Alison B. Loupos1
Estimated H-index: 1
(UD: University of Delaware),
Jack Gelb20
Estimated H-index: 20
(UD: University of Delaware)
Five strains of infectious bronchitis virus isolated from commercial chickens from the state of Pennsylvania, USA during the years 1998 and 1999 were studied. The strains were selected for cross-challenge in specific pathogen free chickens and virus neutralization in chick embryos on the basis of partial S1 sequence amino acid identity values. The partial sequences analysed spanned the hypervariable amino terminus region of S1 from amino acid residues 48 to 219, based on the Beaudette strain. Us...
Published on Jun 1, 2005in Avian Pathology1.96
Jack Gelb20
Estimated H-index: 20
(UD: University of Delaware),
Y. Weisman1
Estimated H-index: 1
+ 1 AuthorsR. Meir1
Estimated H-index: 1
The S1 genes of isolates of avian coronavirus infectious bronchitis virus (IBV) from commercial chickens in the US and Israel (20 isolates from each country) were studied using reverse transcription-polymerase chain reaction restriction fragment length polymorphism and sequencing. Partial sequences spanning the amino terminus region of S1 from amino acid residues 48 to 219, based on the Beaudette strain, were used for analysis. Phylogenetic clustering and high-sequence identity values were used ...
Cited By27
Newest
Published in Molecular and Cellular Probes2.51
Ruy D. Chacón1
Estimated H-index: 1
(USP: University of São Paulo),
Claudete S. Astolfi-Ferreira7
Estimated H-index: 7
(USP: University of São Paulo)
+ -3 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
Abstract Infectious bronchitis (IB) is one of the avian diseases with the greatest impact on poultry farming worldwide. In Brazil, strain BR-I (GI-11) is the most prevalent in poultry flocks. The present study aimed to develop a seminested RT-PCR assay specific for the diagnosis of BR-I IBV in Brazilian samples, targeting subunit 1 of the S gene. The detection limit of this assay was 10 copies of the IBV genome. In this study, 62.24% of 572 organ pools from the 5 regions of Brazil tested positiv...
Published on May 4, 2019in Avian Pathology1.96
Ana Marandino5
Estimated H-index: 5
,
Ariel Vagnozzi12
Estimated H-index: 12
(INTA: International Trademark Association)
+ 5 AuthorsRubén Pérez36
Estimated H-index: 36
ABSTRACTInfectious bronchitis virus (IBV) is a persistent sanitary problem for the South American poultry industry despite extensive vaccination. The IBV single-stranded RNA genome has high rates of mutation and recombination that generate a notorious virus variability. Since most IBV vaccines are type-specific, there is a need for constant surveillance of the circulating lineages and knowledge about their genetic and antigenic properties. Here we present an integrative analysis that provides th...
Published on Jan 1, 2019in Brazilian Journal of Microbiology2.86
Nicole Assis Pereira (USP: University of São Paulo), Antonio Carlos Alessi16
Estimated H-index: 16
(UNESP: Sao Paulo State University)
+ 5 AuthorsLeonardo José Richtzenhain28
Estimated H-index: 28
(USP: University of São Paulo)
An outbreak of infectious bronchitis caused by the IBVPR03 strain of the Massachusetts genotype affected H-120 vaccinated laying hens in South Brazil. We investigated the cross protection of the vaccine by assessing the traqueal ciliostasis, virus recovery, and histopathological changes typically observed in the respiratory tract. Although the IBVPR03 strain is S1-genotyped as Massachusetts with a high genomic similarity to the H-120 vaccine strains, surprisingly, we found no tropism or pathogen...
Published on Jul 1, 2018in Infection, Genetics and Evolution2.61
Aline Padilha Fraga3
Estimated H-index: 3
(ULBRA: Universidade Luterana do Brasil),
Aline Padilha de Fraga1
Estimated H-index: 1
+ 3 AuthorsVagner Ricardo Lunge12
Estimated H-index: 12
(ULBRA: Universidade Luterana do Brasil)
Abstract Avian infectious bronchitis virus (IBV) is the etiological agent of a highly contagious disease, which results in severe economic losses to the poultry industry. The spike protein (S1 subunit) is responsible for the molecular diversity of the virus and many sero/genotypes are described around the world. Recently a new standardized classification of the IBV molecular diversity was conducted, based on phylogenetic analysis of the S1 gene sequences sampled worldwide. Brazil is one of the b...
Published on May 4, 2018in Avian Pathology1.96
Matheus C. Martini4
Estimated H-index: 4
(State University of Campinas),
Leonardo Cardia Caserta4
Estimated H-index: 4
(State University of Campinas)
+ 11 AuthorsJuliana Cristina Santiago Bastos3
Estimated H-index: 3
(State University of Campinas)
ABSTRACTThe detection of avian coronaviruses (AvCoV) in wild birds and the emergence of new AvCoV have increased in the past few years. In the present study, the pathogenicity of three AvCoV isolates was investigated in day-old chicks. One AvCoV isolated from a pigeon, which clustered with the Massachusetts vaccine serotype, and two AvCoV isolated from chickens, which grouped with a Brazilian genotype lineage, were used. Clinical signs, gross lesions, histopathological changes, ciliary activity,...
Published on May 1, 2018in Journal of Comparative Pathology1.25
Nguyen Phuc Khanh1
Estimated H-index: 1
(UPM: Universiti Putra Malaysia),
Sheau Wei Tan12
Estimated H-index: 12
(UPM: Universiti Putra Malaysia)
+ 5 AuthorsAbdul Rahman Omar26
Estimated H-index: 26
(UPM: Universiti Putra Malaysia)
Summary Infectious bronchitis viruses (IBVs) circulating in Malaysia are classified into two groups as Malaysian QX-like and variant strains. In this study, the pathogenicity of IBS130/2015 (QX-like) and IBS037A/2014 (variant) IBVs in 1-day-old and 30-day-old specific pathogen free (SPF) chickens was characterized. Both strains caused respiratory and kidney infections based on immunohistochemistry (IHC), real-time quantitative polymerase chain reaction (qPCR) and a ciliostasis study; however, th...
Published on Feb 1, 2018in Virus Genes1.62
Giuliana Loreto Saraiva2
Estimated H-index: 2
(UFV: Universidade Federal de Viçosa),
Marcus Rebouças Santos3
Estimated H-index: 3
(UFV: Universidade Federal de Viçosa)
+ 7 AuthorsAbelardo Silva-Júnior3
Estimated H-index: 3
(UFV: Universidade Federal de Viçosa)
Infectious bronchitis virus (IBV) is currently one of the most important pathogens in the poultry industry. The H120 and Ma5 are the only viral strains approved by the Brazilian government as the constituent of vaccines. Despite the systematic vaccination in Brazil, IBV has not yet been controlled and diseases associated with this virus have been reported in vaccinated chickens. Here, we investigated the genetic variability of H120 and Ma5 strains present in the IBV vaccines from different Brazi...
Published on Jan 1, 2018in Preventive Veterinary Medicine2.30
Arianne Brown Jordan3
Estimated H-index: 3
(UWI: University of the West Indies),
Jamie Sookhoo2
Estimated H-index: 2
(UWI: University of the West Indies)
+ 5 AuthorsC.A.L. Oura26
Estimated H-index: 26
(UWI: University of the West Indies)
Viruses affecting poultry cause significant levels of disease leading to severe economic losses among poultry farmers worldwide. The Americas region continues to be vulnerable to the spread of poultry viruses across the continents and Caribbean island chains. In Trinidad and Tobago (T&T) there is limited information on the viruses circulating in poultry. Many flock are vulnerable to infection and there are occasional outbreaks of disease resulting in high levels of morbidity and mortality. This ...
Published on Nov 2, 2017in British Poultry Science1.42
Claudia Carranza3
Estimated H-index: 3
(USP: University of São Paulo),
Claudete S. Astolfi-Ferreira7
Estimated H-index: 7
(USP: University of São Paulo)
+ 6 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
1. Infectious bronchitis virus (IBV) variants in Brazil were isolated during 2010-2015 for epidemiological and molecular analysis to characterise the different variants and perform a bioinformatic analysis to compare with sequences of variants collected over the previous 40 years. 2. Of the 453 samples examined, 61.4% were positive for IBV and 75.9% of these were considered to have the BR-I genotype and were detected in birds of all ages distributed in all five Brazilian regions. 3. The ratio of...
Published on Oct 1, 2017in Infection, Genetics and Evolution2.61
Ana Marandino5
Estimated H-index: 5
,
Gonzalo Tomás5
Estimated H-index: 5
+ 6 AuthorsRubén Pérez36
Estimated H-index: 36
Abstract Infectious bronchitis virus ( Gammacoronavirus , Coronaviridae ) is a genetically variable RNA virus that causes one of the most persistent respiratory diseases in poultry. The virus is classified in genotypes and lineages with different epidemiological relevance. Two lineages of the GI genotype (11 and 16) have been widely circulating for decades in South America. GI-11 is an exclusive South American lineage while the GI-16 lineage is distributed in Asia, Europe and South America. Here...