Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells.
Abstract
We report here homologous recombination (HR)-mediated gene targeting of two different genes in human iPS cells (hiPSCs) and human ES cells (hESCs). HR-mediated correction of a chromosomally integrated mutant GFP reporter gene reaches efficiencies of 0.14%-0.24% in both cell types transfected by donor DNA with plasmids expressing zinc finger nucleases (ZFNs). Engineered ZFNs that induce a sequence-specific double-strand break in the GFP gene...
Paper Details
Title
Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells.
Published Date
Jul 2, 2009
Journal
Volume
5
Issue
1
Pages
97 - 110
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History