Cryocrystallography in capillaries: critical glycerol concentrations and cooling rates

Capillary tubes have many advantages over multi-well plates for macromolecular crystal growth and handling, including the possibility of in situ structure determination. To obtain complete high-resolution X-ray data sets, cryopreservation protocols must be developed to prevent crystalline ice formation and preserve macromolecular crystal order. The minimum glycerol concentrations required to vitrify aqueous solutions during plunging into liquid...