Effective approaches for the production of heterologous proteins using the Thermococcus kodakaraensis-based translation system

Volume: 133, Issue: 2, Pages: 177 - 182
Published: Jan 20, 2008
Abstract
We have previously established a system for cell-free protein synthesis that can be operated at high temperatures using the cell lysate of a hyperthermophilic archaeon, Thermococcus kodakaraensis. To apply this system to practical use in the field of heterologous protein production, the performance of our system in the synthesis of green fluorescent protein (GFP) was examined. As the wild-type GFP is a thermolabile protein, a thermostable GFP...
Paper Details
Title
Effective approaches for the production of heterologous proteins using the Thermococcus kodakaraensis-based translation system
Published Date
Jan 20, 2008
Volume
133
Issue
2
Pages
177 - 182
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