Development and validation of nested-PCR for the diagnosis of clinical and subclinical infectious laryngotracheitis

A standardised nested-PCR method that amplifies a region of the glycoprotein E gene of avian infectious laryngotracheitis virus (ILTV) has been developed for the diagnosis of infection by Gallid herpesvirus 1. The two sets of primers employed produced the expected amplification products of 524 bp (external primers) and 219 bp (internal primers) in the presence of ILTV DNA, whereas no such amplicons were obtained with other avian respiratory...