Mutagenesis of Klebsiella aerogenes UreG To Probe Nickel Binding and Interactions with Other Urease-Related Proteins

UreG is a GTPase required for assembly of the nickel-containing active site of urease. Herein, a Strep-tagged Klebsiella aerogenes UreG (UreGStr) and selected site-directed variants of UreGStr were constructed for studying the in vivo effects on urease activation in recombinant Escherichia coli cells, characterizing properties of the purified proteins, and analysis of in vivo and in vitro protein−protein interactions. Whereas the Strep tag had...