Strategy for comprehensive identification of humanN-myristoylated proteins using an insect cell-free protein synthesis system
Abstract
To establish a strategy for the comprehensive identification of human N-myristoylated proteins, the susceptibility of human cDNA clones to protein N-myristoylation was evaluated by metabolic labeling and MS analyses of proteins expressed in an insect cell-free protein synthesis system. One-hundred-and-forty-one cDNA clones with N-terminal Met-Gly motifs were selected as potential candidates from approximately 2000 Kazusa ORFeome project human...
Paper Details
Title
Strategy for comprehensive identification of humanN-myristoylated proteins using an insect cell-free protein synthesis system
Published Date
Apr 28, 2010
Journal
Volume
10
Issue
9
Pages
1780 - 1793
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