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In Vivo Evidence that S-Adenosylmethionine and Fatty Acid Synthesis Intermediates Are the Substrates for the LuxI Family of Autoinducer Synthases

Published on May 1, 1998in Journal of Bacteriology3.23
Dale L. Val6
Estimated H-index: 6
,
John E. Cronan73
Estimated H-index: 73
(UIUC: University of Illinois at Urbana–Champaign)
Abstract
Many gram-negative bacteria synthesize N-acyl homoserine lactone autoinducer molecules as quorum-sensing signals which act as cell density-dependent regulators of gene expression. We have investigated the in vivo source of the acyl chain and homoserine lactone components of the autoinducer synthesized by the LuxI homolog, TraI. In Escherichia coli, synthesis of N-(3-oxooctanoyl)homoserine lactone by TraI was unaffected in a fadD mutant blocked in β-oxidative fatty acid degradation. Also, conditions known to induce the fad regulon did not increase autoinducer synthesis. In contrast, cerulenin and diazoborine, specific inhibitors of fatty acid synthesis, both blocked autoinducer synthesis even in a strain dependent on β-oxidative fatty acid degradation for growth. These data provide the first in vivo evidence that the acyl chains in autoinducers synthesized by LuxI-family synthases are derived from acyl-acyl carrier protein substrates rather than acyl coenzyme A substrates. Also, we show that decreased levels of intracellular S-adenosylmethionine caused by expression of bacteriophage T3 S-adenosylmethionine hydrolase result in a marked reduction in autoinducer synthesis, thus providing direct in vivo evidence that the homoserine lactone ring of LuxI-family autoinducers is derived from S-adenosylmethionine.
  • References (65)
  • Citations (114)
References65
Newest
Published on Mar 3, 2005in FEBS Journal4.74
Peter Overath48
Estimated H-index: 48
,
G. Pauli1
Estimated H-index: 1
,
H. U. Schairer1
Estimated H-index: 1
1 An enzymatic activity is described in oleate-grown cells of E. coli, which has the cofactor requirements of a long chain acid: CoA ligase (AMP). This enzyme is at least partially membrane bound and activates saturated and mono- and polyunsaturated fatty acids. It is inactive with butyrate and hexanoate. The enzyme is induced by oleate under similar conditions, but at different relative rates, as three other enzymes of fatty acid degradation (thiolase, crotonase, hydroxyacyl-CoA dehydrogenase) ...
Published on Jan 1, 1989
Joseph Sambrook29
Estimated H-index: 29
,
Edward F. Fritsch16
Estimated H-index: 16
,
Tom Maniatis126
Estimated H-index: 126
Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefe...
Paul D. Shaw17
Estimated H-index: 17
(UIUC: University of Illinois at Urbana–Champaign),
Gao Ping1
Estimated H-index: 1
(UIUC: University of Illinois at Urbana–Champaign)
+ 4 AuthorsStephen K. Farrand41
Estimated H-index: 41
(UIUC: University of Illinois at Urbana–Champaign)
Many Gram-negative bacteria regulate gene expression in response to their population size by sensing the level of acyl-homoserine lactone signal molecules which they produce and liberate to the environment. We have developed an assay for these signals that couples separation by thin-layer chromatography with detection using Agrobacterium tumefaciens harboring lacZ fused to a gene that is regulated by autoinduction. With the exception of N-butanoyl-l-homoserine lactone, the reporter detected acyl...
Published on Oct 1, 1996in Annual Review of Microbiology10.24
Clay Fuqua25
Estimated H-index: 25
(Trinity University),
Stephen C. Winans46
Estimated H-index: 46
(Cornell University),
E. P. Greenberg83
Estimated H-index: 83
(UI: University of Iowa)
▪ Abstract The importance of accurate demographic information is reflected in the United States Constitution, Article 1, which provides for a decennial census of this country's human population. Bacteria also conduct a census of their population and do so more frequently, more efficiently, and as far we know, with little if any of the political contentiousness caused by human demographers. Many examples have been found of particular bacterial genes, operons, or regulons that are expressed prefer...
Amy L. Schaefer22
Estimated H-index: 22
(UI: University of Iowa),
Dale L. Val6
Estimated H-index: 6
(UIUC: University of Illinois at Urbana–Champaign)
+ 2 AuthorsE. P. Greenberg83
Estimated H-index: 83
(UI: University of Iowa)
Abstract Many bacteria use acyl homoserine lactone signals to monitor cell density in a type of gene regulation termed quorum sensing and response. Synthesis of these signals is directed by homologs of the luxi gene of Vibrio fischeri. This communication resolves two critical issues concerning the synthesis of the V. fischeri signal. (i) The luxI product is directly involved in signal synthesis-the protein is an acyl homoserine lactone synthase; and (ii) the substrates for acyl homoserine lacton...
Published on Sep 1, 1996in Journal of Bacteriology3.23
Brian L. Hanzelka8
Estimated H-index: 8
(UI: University of Iowa),
E. P. Greenberg83
Estimated H-index: 83
(UI: University of Iowa)
Synthesis of the autoinducer signal involved in the cell density-dependent activation of Vibrio fischeri luminescence is directed by luxI. The autoinducer is N-(3-oxohexanoyl)homoserine lactone, and little is known about its synthesis. We have measured autoinducer synthesis by amino acid auxotrophs of Escherichia coli that contained luxI on a high-copy-number plasmid. Experiments with cell suspensions starved for methionine or homoserine show that either methionine or S-adenosylmethionine but no...
Published on Jul 1, 1996in Journal of Molecular Biology5.07
Bruno Miroux3
Estimated H-index: 3
(LMB: Laboratory of Molecular Biology),
John Wrightson86
Estimated H-index: 86
(LMB: Laboratory of Molecular Biology)
Abstract We have investigated the over-production of seven membrane proteins in an Escherichia coli – bacteriophage T7 RNA polymerase expression system. In all seven cases, when expression of the target membrane protein was induced, most of the BL21(DE3) host cells died. Similar effects were also observed with expression vectors for ten globular proteins. Therefore, protein over-production in this expression system is either limited or prevented by bacterial cell death. From the few survivors of...
Published on Jun 14, 1996in Science41.04
Margret I. Moré5
Estimated H-index: 5
(Cornell University),
Finger Ld1
Estimated H-index: 1
(Ithaca College)
+ 3 AuthorsStephen C. Winans46
Estimated H-index: 46
(Cornell University)
Many bacteria, including several pathogens of plants and humans, use a pheromone called an autoinducer to regulate gene expression in a cell density-dependent manner. Agrobacterium autoinducer [AAI, N -(3-oxo-octanoyl)-L-homoserine lactone] of A. tumefaciens is synthesized by the TraI protein, which is encoded by the tumor-inducing plasmid. Purified hexahistidinyl-TraI (H 6 -TraI) used S -adenosylmethionine to make the homoserine lactone moiety of AAI, but did not use related compounds. H 6 -Tra...
Published on Apr 1, 1996in Molecular Microbiology3.65
Leo Eberl72
Estimated H-index: 72
(DTU: Technical University of Denmark),
Michael K. Winson24
Estimated H-index: 24
(University of Nottingham)
+ 7 AuthorsMichaelGIVSKOV95
Estimated H-index: 95
(DTU: Technical University of Denmark)
Summary Several bacterial species possess the ability to differentiate into highly motile swarmer cells capable of rapid surface colonization. In Serratia liquefaciens, we demonstrate that initiation of swarmer-cell differentiation involves diffusible signal molecules that are released into the growth medium. Using high-performance liquid chromatography (HPLC), high resolution mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy, we identified N-butanoyl-l-homoserine lactone (BHL)...
Published on Jan 1, 1996in Gene2.64
Natalie D. Fedorova1
Estimated H-index: 1
(EIMB: Engelhardt Institute of Molecular Biology),
Mikhail Y. Peredelchuk1
Estimated H-index: 1
(Rice University)
+ 1 AuthorsGeorge N. Bennett65
Estimated H-index: 65
(Rice University)
Abstract An Escherichia coli strain for thermoinducible T7Pol-driven transcription has been constructed. The strain was obtained by site-specific integration of the T7 gene 1 coding for T7Po1 into the attB site of phage λ in the E. coli chromosome. The expression of the inserted gene is regulated by cIts857 and major early promoter-operator regions of phage λ.
Cited By114
Newest
Published on May 8, 2018in Mbio6.75
Zhe Hu5
Estimated H-index: 5
(SCAU: South China Agricultural University),
Huijuan Dong2
Estimated H-index: 2
(SCAU: South China Agricultural University)
+ 8 AuthorsJohn E. Cronan22
Estimated H-index: 22
(UIUC: University of Illinois at Urbana–Champaign)
Published on Jan 1, 2018
Daniel Shin2
Estimated H-index: 2
(BSU: Boise State University),
Rajesh Nagarajan8
Estimated H-index: 8
(BSU: Boise State University)
Published on Jan 1, 2018
Basit Yousuf (Kyushu University), Keika Adachi1
Estimated H-index: 1
(Kyushu University),
Jiro Nakayama36
Estimated H-index: 36
(Kyushu University)
Quorum sensing is a process of chemical communication that adjusts the genetic expression of certain important biological functions in a cell-density—dependent manner. Quorum sensing (QS) regulates important bacterial behaviors, such as production of virulence factors, formation of biofilm, and antibiotic resistance, via signaling molecules called autoinducers (AIs). Gram-negative bacteria typically use N-acyl homoserine lactones (AHLs) and S-adenosyl methionine (SAM) molecules as signaling mole...
Published on Oct 1, 2017in Journal of Microbiology2.32
Xi-Hui Li4
Estimated H-index: 4
(PNU: Pusan National University),
Joon-Hee Lee13
Estimated H-index: 13
(PNU: Pusan National University)
Biofilms are complex microbial architectures that attach to surfaces and encase microorganisms in a matrix composed of self-produced hydrated extracellular polymeric substances (EPSs). In biofilms, microorganisms become much more resistant to antimicrobial treatments, harsh environmental conditions, and host immunity. Biofilm formation by microbial pathogens greatly enhances survival in hosts and causes chronic infections that result in persistent inflammation and tissue damages. Currently, it i...
Published on Dec 1, 2016in Scientific Reports4.01
Yong-Hong Yu3
Estimated H-index: 3
,
Zhe Hu5
Estimated H-index: 5
+ 2 AuthorsHaihong Wang9
Estimated H-index: 9
Xanthomonas campestris FabH is required for branched-chain fatty acid and DSF-family quorum sensing signal biosynthesis
Published on Nov 10, 2016in PLOS ONE2.78
Chih-Yuan Hsu1
Estimated H-index: 1
,
Bor-Sen Chen1
Estimated H-index: 1
With the recent industrial expansion, heavy metals and other pollutants have increasingly contaminated our living surroundings. Heavy metals, being non-degradable, tend to accumulate in the food chain, resulting in potentially damaging toxicity to organisms. Thus, techniques to detect metal ions have gradually begun to receive attention. Recent progress in research on synthetic biology offers an alternative means for metal ion detection via the help of promoter elements derived from microorganis...
Published on Sep 1, 2016in Water Air and Soil Pollution1.77
Merlin Antony1
Estimated H-index: 1
(Mahatma Gandhi University),
K. Jayachandran1
Estimated H-index: 1
(Mahatma Gandhi University)
The cell density-dependent gene expression in gram-negative bacteria is through the activity of acyl homoserine lactone signal molecules. The novel strain Pseudomonas putida JMQS1 isolated from detergent-contaminated soil exhibited quorum sensing along with its ability to degrade phenol. The response to Chromobacterium violaceum DSTS-1 mutant biosensor and luxI and luxR gene-specific amplification was used to characterize the quorum sensing property of the isolate. A regulation in the synthesis ...
Published on Aug 1, 2016in Chemosphere5.11
Jinhui Huang24
Estimated H-index: 24
(Hunan University),
Yahui Shi8
Estimated H-index: 8
(Hunan University)
+ 6 AuthorsJianxin Zhou4
Estimated H-index: 4
(Hunan University)
Abstract Quorum sensing (QS) is a communication process between cells, in which bacteria secrete and sense the specific chemicals, and regulate gene expression in response to population density. Quorum quenching (QQ) blocks QS system, and inhibits gene expression mediating bacterial behaviors. Given the extensive research of acyl-homoserine lactone (AHL) signals, existences and effects of AHL-based QS and QQ in biological wastewater treatments are being subject to high concern. This review summa...
Published on Aug 1, 2016in Acta Biochimica et Biophysica Sinica2.50
Manluan Sun2
Estimated H-index: 2
(CAS: Chinese Academy of Sciences),
Hongsen Guo1
Estimated H-index: 1
(HAU: Huazhong Agricultural University)
+ 4 AuthorsJiao-Yu Deng20
Estimated H-index: 20
(CAS: Chinese Academy of Sciences)
Lysine acetylation is one of the most abundant post-translational modifications. However, physiological roles of this modification in bacteria are largely unknown. Previous protein acetylome analysis showed that Escherichia coli adenosylmethionine synthase (MAT) undergoes acetylation in vivo, but the biological functions of this modification still need to be uncovered. In this study, MAT of E. coli was over-expressed and purified. Subsequent mass spectrometry analysis showed that 12 lysine resid...