CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

An sgRNA-scoring algorithm (CRISPRscan) based on molecular features that enhance activity allows users to predict the most efficient sgRNA for in vivo targets. CRISPR-Cas9 technology provides a powerful system for genome engineering. However, variable activity across different single guide RNAs (sgRNAs) remains a significant limitation. We analyzed the molecular features that influence sgRNA stability, activity and loading into Cas9 in vivo. We...