Original paper
Intermediate-sensor assisted push–pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli
Abstract
Because high-throughput screening tools are typically unavailable when using the pathway-engineering approach, we developed a new strategy, named intermediate sensor-assisted push-pull strategy, which enables sequential pathway optimization by incorporating a biosensor targeting a key pathway intermediate. As proof of concept, we constructed an L-Trp biosensor and used it to optimize the deoxyviolacein biosynthetic pathway, which we divided into...
Paper Details
Title
Intermediate-sensor assisted push–pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli
Published Date
Jan 1, 2016
Journal
Volume
33
Pages
41 - 51
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Notes
History