Branding/Logomark minus Citation Combined Shape Icon/Bookmark-empty Icon/Copy Icon/Collection Icon/Close Copy 7 no author result Created with Sketch. Icon/Back Created with Sketch. Match!

A novel aptasensor based on single-molecule force spectroscopy for highly sensitive detection of mercury ions

Published on Jan 1, 2015in Analyst 4.02
· DOI :10.1039/C5AN00708A
Qing Li9
Estimated H-index: 9
(University of Bremen),
Monika Michaelis2
Estimated H-index: 2
(University of Bremen)
+ 1 AuthorsLucio Colombi Ciacchi20
Estimated H-index: 20
(University of Bremen)
Cite
Abstract
We have developed a novel aptasensor based on single-molecule force spectroscopy (SMFS) capable of detecting mercury ions (Hg2+) with sub-nM sensitivity. The single-strand (ss) DNA aptamer used in this work is rich in thymine (T) and readily forms T–Hg2+–T complexes in the presence of Hg2+. The aptamer was conjugated to an atomic force microscope (AFM) probe, and the adhesion force between the probe and a flat graphite surface was measured by single-molecule force spectroscopy (SMFS). The presence of Hg2+ ions above a concentration threshold corresponding to the affinity constant of the ions for the aptamer (about 5 × 109 M−1) could be easily detected by a change of the measured adhesion force. With our chosen aptamer, we could reach an Hg2+ detection limit of 100 pM, which is well below the maximum allowable level of Hg2+ in drinking water. In addition, this aptasensor presents a very high selectivity for Hg2+ over other metal cations, such as K+, Ca2+, Zn2+, Fe2+, and Cd2+. Furthermore, the effects of the ionic strength and loading rate on the Hg2+ detection were evaluated. Its simplicity, reproducibility, high selectivity and sensitivity make our SMFS-based aptasensor advantageous with respect to other current Hg2+ sensing methods. It is expected that our strategy can be exploited for monitoring the pollution of water environments and the safety of potentially contaminated food.
  • References (39)
  • Citations (17)
Cite
References39
Newest
Published on Mar 19, 2015in Biosensors
Jingfeng Li16
Estimated H-index: 16
(University of Bremen),
Qing Li9
Estimated H-index: 9
(University of Bremen)
+ 1 AuthorsGang Wei36
Estimated H-index: 36
(University of Bremen)
We demonstrate a simple force-based label-free strategy for the highly sensitive sensing of adenosine. An adenosine ssDNA aptamer was bound onto an atomic force microscopy (AFM) probe by covalent modification, and the molecular-interface adsorption force between the aptamer and a flat graphite surface was measured by single-molecule force spectroscopy (SMFS). In the presence of adenosine, the molecular recognition between adenosine and the aptamer resulted in the formation of a folded, hairpin-l...
Published on Jan 1, 2014in Physical Chemistry Chemical Physics 3.57
Gang Wei36
Estimated H-index: 36
(University of Bremen),
Qing Li9
Estimated H-index: 9
(University of Bremen)
+ 1 AuthorsLucio Colombi Ciacchi20
Estimated H-index: 20
(University of Bremen)
We report here a systematic investigation of the interactions between two heteropolymer DNA single-strands (ssDNA) and graphite surfaces using AFM-based single-molecule force spectroscopy (SMFS). For this purpose, force-displacement (FD) curves are recorded by peeling single-molecule ssDNA from graphite. We find that the unbinding forces are affected both by the DNA sequences and the ionic strength of the liquid environment. In particular, the unbinding force decreases with the increase of ionic...
Published on Aug 13, 2013in Langmuir 3.68
Yair Razvag6
Estimated H-index: 6
,
Vitaly Gutkin14
Estimated H-index: 14
,
Meital Reches24
Estimated H-index: 24
This article describes single-molecule force spectroscopy measurements of the interaction between individual amino acid residues and inorganic surfaces in an aqueous solution. In each measurement, there is an amino acid residue, lysine, glutamate, phenylalanine, leucine, or glutamine, and each represents a class of amino acids (positively or negatively charged, aromatic, nonpolar, and polar). Force–distance curves measured the interaction of the individual amino acid bound to a silicon atomic fo...
Published on May 28, 2013in ACS Nano 13.90
Ling Zhang33
Estimated H-index: 33
,
Haixin Chang12
Estimated H-index: 12
+ 3 AuthorsMingwei Chen82
Estimated H-index: 82
Precisely probing heavy metal ions in water is important for molecular biology, environmental protection, and healthy monitoring. Although many methods have been reported in the past decade, developing a quantitative approach capable of detecting sub-ppt level heavy metal ions with high selectivity is still challenging. Here we report an extremely sensitive and highly selective nanoporous gold/aptamer based surface enhanced resonance Raman scattering (SERRS) sensor. The optical sensor has an unp...
Published on Apr 1, 2013in Electroanalysis 2.69
Etery Sharon16
Estimated H-index: 16
(HUJI: Hebrew University of Jerusalem),
Xiaoqing Liu34
Estimated H-index: 34
(HUJI: Hebrew University of Jerusalem)
+ 2 AuthorsItamar Willner120
Estimated H-index: 120
(HUJI: Hebrew University of Jerusalem)
Graphene oxide (GO) is deposited onto the Al2O3 gates of field-effect transistor devices to yield a functional matrix for the sensing of thrombin or Hg2+ ions. The deposition of an aptamer subunit against thrombin or a tailored nucleic acid with appropriate T mismatches on the GO, yield active surfaces for the analysis of thrombin or Hg2+ ions. The desorption of the aptamer-subunit from GO, through the formation of the aptamer-thrombin complex, or the desorption of the T–Hg2+–T-bridged duplex DN...
Published on Mar 1, 2013in Biosensors and Bioelectronics 9.52
Ming Li27
Estimated H-index: 27
(WVU: West Virginia University),
Xuejiao Zhou18
Estimated H-index: 18
(SJTU: Shanghai Jiao Tong University)
+ 2 AuthorsNianqiang Wu58
Estimated H-index: 58
(WVU: West Virginia University)
Abstract Label-free fluorescent detection of Hg 2+ has been realized via quenching of fluorescence of graphene oxide (GO). The water-soluble GO sheets, which are functionalized with single-stranded DNA aptamer, exhibit strong fluorescence emission at 600 nm under the excitation of 488 nm in the absence of Hg 2+ ions. When Hg 2+ ions appear in the aqueous solution, Hg 2+ ions are sandwiched between the hairpin-shaped double-stranded DNA due to the formation of the thymine–Hg 2+ –thymine complex, ...
Published on Jan 1, 2013in New Journal of Chemistry 3.07
Wenping Zhu7
Estimated H-index: 7
(Hunan University),
Ziwei Zhao4
Estimated H-index: 4
(Hunan University)
+ 4 AuthorsRu-Qin Yu72
Estimated H-index: 72
(Hunan University)
A label free exonuclease III (Exo III)-aided fluorescence assay for adenosine triphosphate (ATP) was developed based on the ATP-dependent enzymatic reaction and graphene oxide (GO). This strategy relies on the principle that Exo III shows different cleavage capacity for a DNA substrate in the absence and presence of ATP and the preferential binding of GO to single-stranded DNA over double-stranded one. By combining the unique properties of SYBR Green I and GO adsorption, this sensor displays an ...
Published on Jan 1, 2013in Chemical Communications 6.16
Gang Wei36
Estimated H-index: 36
(University of Bremen),
Sascha Steckbeck4
Estimated H-index: 4
(University of Bremen)
+ 1 AuthorsLucio Colombi Ciacchi20
Estimated H-index: 20
(Fraunhofer Society)
We demonstrate here a novel single-molecule, label-free bioanalytical system capable of sensing the presence of specific ssDNA oligomer sequences and proteins with high selectivity and sensitivity. An ssDNA concentration of 1 nM and a Lyz concentration of 0.65 nM could be detected.
Published on Jul 5, 2012in Journal of Physical Chemistry C 4.31
Sara Iliafar3
Estimated H-index: 3
,
Kyle Wagner4
Estimated H-index: 4
+ 2 AuthorsDmitri V. Vezenov21
Estimated H-index: 21
In single molecule force spectroscopy experiments, force probes chemically modified with synthetic, single-stranded DNA oligomers produced characteristic steady-state forces connected by abrupt steps between plateaus, as the probes moved away from a graphite substrate. The force plateaus represent peeling of a small number of polymer molecules from the flat surface. The final force jump in the retraction region of the force–distance curves can be attributed to a single DNA molecule detaching fro...
Published on Apr 25, 2012in ACS Applied Materials & Interfaces 8.46
Youssef Helwa3
Estimated H-index: 3
,
Neeshma Dave13
Estimated H-index: 13
+ 2 AuthorsJuewen Liu59
Estimated H-index: 59
With a low optical background, high loading capacity, and good biocompatibility, hydrogels are ideal materials for immobilization of biopolymers to develop optical biosensors. We recently immobilized mercury and lead binding DNAs within a monolithic gel and demonstrated ultrasensitive visual detection of these heavy metals. The high sensitivity was attributed to the enrichment of the analytes into the gels. The signaling kinetics was slow, however, taking about 1 h to obtain a stable optical sig...
Cited By17
Newest
Published on Aug 1, 2019in Talanta 4.92
Yan Gong13
Estimated H-index: 13
(Xi'an Jiaotong University),
Yamin Zheng1
Estimated H-index: 1
(Xi'an Jiaotong University)
+ 6 AuthorsFei Li29
Estimated H-index: 29
(Xi'an Jiaotong University)
Abstract Upconversion nanoparticle-based lateral flow assays (UCNP-LFAs) have attracted significant attention in point-of-care testing (POCT) applications, due to the long-term photostability and enhanced signal-to-background noise ratio. The existing UCNP-LFAs generally require peripheral equipment for exciting fluorescent signals and reading out fluorescence results, which are generally bulky and expensive. Herein, we developed a miniaturized and portable UCNP-LFA platform, which is composed o...
Published on Jun 1, 2019in International Journal of Biological Macromolecules 4.78
Tiago Augusto da Silva Moura2
Estimated H-index: 2
(UFV: Universidade Federal de Viçosa),
Leandro Licursi de Oliveira15
Estimated H-index: 15
(UFV: Universidade Federal de Viçosa),
M. S. Rocha13
Estimated H-index: 13
(UFV: Universidade Federal de Viçosa)
Abstract Here, we use single molecule force spectroscopy performed with optical tweezers in order to investigate the interaction between Caffeine and the DNA molecule for various different concentrations of the alkaloid and under two distinct ionic strengths of the surrounding buffer. We were able to determine the mechanical changes induced on the double-helix structure due to Caffeine binding, the binding mode and the binding parameters of the interaction. The results obtained show that Caffein...
Published on Jan 1, 2019in Nanoscale 6.97
Coucong Gong2
Estimated H-index: 2
(University of Bremen),
Shuwei Sun1
Estimated H-index: 1
(Huada: Beijing University of Chemical Technology)
+ 4 AuthorsGang Wei36
Estimated H-index: 36
(CAS: Chinese Academy of Sciences)
Bioinspired synthesis offers potential green strategies to build highly complex nanomaterials by utilizing the unique nanostructures, functions, and properties of biomolecules, in which the biomolecular recognition and self-assembly processes play important roles in tailoring the structures and functions of bioinspired materials. Further understanding of biomolecular self-assembly for inspiring the formation and assembly of nanoparticles would promote the design and fabrication of functional nan...
Published on Jan 1, 2019in Trends in Food Science and Technology 8.52
R. Alexander Reese1
Estimated H-index: 1
(UGA: University of Georgia),
Bingqian Xu24
Estimated H-index: 24
(UGA: University of Georgia)
Abstract Background Food safety is vital in everyday life. Food toxins are small peptides or proteins that can cause disease by disrupting biological macromolecules such as enzymes or cellular receptors. Toxins are introduced either externally or from internal, spoilage-related pathogens. To keep these unsafe foods off the market, detection techniques for various toxins have been devised. Although many techniques serve this purpose well, challenges remain regarding sensitivity, specificity, and ...
Published on Jan 1, 2019in Analytical Methods 2.38
Jingfeng Li16
Estimated H-index: 16
(University of Bremen),
Qing Li9
Estimated H-index: 9
(University of Bremen)
+ 2 AuthorsColombi Ciacchi Lucio (University of Bremen)
Atomic-force-microscopy-based single-molecule force spectroscopy (AFM-SMFS) has become an important technique as the basis of novel, label-free biosensing strategies. In this work, we explored the possibility of using AFM-SMFS to detect the specific peptide cleavage activity by thrombin proteases. To achieve this aim, an oligopeptide with a sequence that is recognized and cleaved by thrombin was spanned between an AFM tip and a solid surface via a PEG-based linker system including the stable bio...
Published on Dec 1, 2018in Sensors and Actuators B-chemical 6.39
Birui Jin3
Estimated H-index: 3
(Xi'an Jiaotong University),
Yexin Yang1
Estimated H-index: 1
(Xi'an Jiaotong University)
+ 7 AuthorsMin Lin19
Estimated H-index: 19
(Xi'an Jiaotong University)
Abstract Simultaneous detection of multiple targets with different analyte-recognition reactions in the same sample in a rapid, low-cost and reliable way has immense potential with widespread applications in food safety, medical diagnostics and environmental monitoring. Herein, we developed a lateral flow aptamer assay (LFAA) integrated smartphone-based portable device for highly sensitive and precise detection of multiple targets, using aptamers functionalized multi-colored upconversion nanopar...
Published on Oct 26, 2018
Annalisa De Girolamo16
Estimated H-index: 16
(National Research Council),
Maureen McKeague13
Estimated H-index: 13
(ETH Zurich)
+ 2 AuthorsMaria C. DeRosa28
Estimated H-index: 28
(Carleton University)
Published on Jan 1, 2018in Physical Chemistry Chemical Physics 3.57
Nils Hildebrand3
Estimated H-index: 3
(University of Bremen),
Gang Wei36
Estimated H-index: 36
(University of Bremen)
+ 1 AuthorsLucio Colombi Ciacchi20
Estimated H-index: 20
(University of Bremen)
The force spectra of proteins detaching from oxide surfaces measured by atomic force microscopy (AFM) often present complex patterns of peaks, which are difficult to correlate with individual bond-breaking events at the atomic scale. In this work we rationalize experimental AFM force spectra of hen-egg-white lysozyme detaching from silica by means of all-atom steered molecular dynamics (SMD) simulations. In particular, we demonstrate that the native tertiary structure of lysozyme is preserved if...
Published on Jan 1, 2018in Analyst 4.02
Li Wang4
Estimated H-index: 4
(Jilin Normal University),
Aiguo Wu34
Estimated H-index: 34
(CAS: Chinese Academy of Sciences),
Gang Wei36
Estimated H-index: 36
(CAS: Chinese Academy of Sciences)
Graphene-based nanomaterials have been widely utilized to fabricate various biosensors for environmental monitoring, food safety, and biomedical diagnostics. The combination of aptamers with graphene for creating biofunctional nanocomposites improved the sensitivity and selectivity of fabricated biosensors due to the unique molecular recognition and biocompatibility of aptamers. In this review, we highlight recent advances in the design, fabrication, and biomedical sensing application of graphen...